Cloning and expression of hygromycin resistance gene (hph) from Streptomyces to Lactobacillus acidophilus: A new clone candidate as an additive to livestock food.
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 2
Abstract
ABSTRACT About 65 local Streptomyces isolates were tested for the presence of hygromycin gene using a rapid PCR dependent method utilizing specific primers designed for this purpose. Only three isolates were found to harbor such gene, and one of them that showed high biological activity was selected for resistance gene (hph) isolation and sequencing. The sequence was similar to that on the expression vector pTKIP-hph which was used to transform Lactobacillus acidophilus with (hph). The transformation efficiency was 0.9 x 106, and this bacterium was able to grow on media with 100μg/ml hygromycin as a selective agent. Real-Time PCR amplification showed that (hph) gene was expressed with high efficiency in the transform ants.
Authors and Affiliations
Rebah N. Algafari
Keywords
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