Co-production of Pectinase and Biosurfactant by the Newly Isolated Strain Bacillus subtilis BKDS1
Journal Title: Microbiology Research Journal International - Year 2015, Vol 10, Issue 2
Abstract
Aim: To isolate pectinase producing bacterial strains from various samples and to investigate the co-production of pectinase and biosurfactant by this isolated strain. Study Design: Isolation, screening, selection and identification of pectinolytic bacteria. Production of pectinase and analysis of exo-pectinase types. Partial purification by ammonium sulphate precipitation and dialysis. The study also analyses the capability of the selected strain for biosurfactant production. Place and Duration of Study: Department of Life Sciences, University of Calicut, Kerala - 673635, India, between July 2013 and November 2014. Methodology: The pectinolytic bacteria was isolated and screened by iodine plate assay method. The enzyme production was confirmed by 3,5-dinitrosalicylic acid (DNS) method. The potent enzyme producing strain was biochemically characterised and identified by 16S rRNA gene sequence analysis. Thiobarbituric acid (TBA) and DNS assay were performed to test the types of exo-pectinase produced. Partial purification of the enzyme was done by ammonium sulphate precipitation. Biosurfactant production by the strain was tested by methods like foam formation, drop collapse test, oil displacement test, microplate assay, hemolytic assay, penetration assay, emulsification activity and bacterial adhesion to hydrocarbon (BATH) assay. Results: Thirty six pectinolytic bacterial strains were screened from the collected samples. Four isolates were selected on the basis of zone size (20 mm to 26 mm) in well plate screening method. In which, the isolate showed maximum enzyme production in DNS assay (0.707 U/mL) is the same which exhibited larger pectin depolymerization area (26 mm) in well diffusion assay. This strain was identified as Bacillus subtilis BKDS1 using 16S rDNA based molecular technique. The optimum incubation time was found to be 72 h for the maximum enzyme production (1.288 U/mL). Assay of exo-pectinase revealed that, the organism was able to produce polygalacturonase (PG), pectin lyase (PNL) and pectate lyase (PEL). In ammonium sulphate precipitation, the enzyme activity was found in 40 -100% salt saturation fractions. On further analysis, it was observed that the organism also produced biosurfactant along with pectinase in the same culture medium. Conclusion: The present study indicates the possibility of an integrated process for obtaining pectinase enzymes and biosurfactants in the same culture media. So this could greatly increase the economic viability of the isolated strain.
Authors and Affiliations
Bijesh Kavuthodi, Steni K. Thomas, Denoj Sebastian
Keywords
Related Articles
- EP ID EP354661
- DOI 10.9734/BMRJ/2015/19627
- Views 56
- Downloads 0
Silver Nanoparticles: Biosynthesis, Characterization and Application on Cotton Fabrics
Aim: Silver nanoparticles are applied to textiles for their strong antimicrobial activity against human pathogenic bacteria which attached at clothes; improve the clinical dressing and potential uses in various medical a...
Prevalence and Antibiotic Susceptibility of Gram-negative Aerobic Bacteria Cultured from the Intestine and Hepatopancreas of Blue Land Crab (Cardisoma guanhumi) in Grenada, West Indies
Aim: To evaluate the prevalence of aerobic bacteria associated with the intestine and hepatopancreas of blue land crab and the susceptibility of the bacteria to a panel of antimicrobials that included some drugs used for...
Bacteremia in Children Infected with HIV/AIDS in Minna, Niger State, Nigeria
Bacterial infection was reported to be one of the leading causes of high mortality among children. Bacteremia is a frequent complication found among HIV infected children and usually associated with a poor prognosis. Thi...
A Preliminary Study on Molecular Characterization of Mycobacterium tuberculosis in Benishangul Gumuz Region, Western Ethiopia
Background: The information about the genetic diversity of Mycobacterium tuberculosis in certain geographic area is needed for a better understanding of epidemiology of tuberculosis and could have implications for develo...
Mycodegradation of Crude Petroleum Oil by Locally Marine Candida tropicalis
Aims: A great demand for crude oil as a source of energy has resulted in an increase in its production, which in turn a gross pollution of the environment. In this study, isolation of local marine yeast with a high pote...