Coexistence of quinolone resistance and extended spectrum beta lactamase production in urinary isolates of Escherichia coli - an emerging challenge to antimicrobial prescribing pattern
Journal Title: Indian Journal of Microbiology Research - Year 2016, Vol 3, Issue 4
Abstract
Background: Urinary tract infections are the second most common infections in community practice and Escherichia coli is the most commonly isolated organism in community acquired UTI. Fluoroquinolones are the preferred antimicrobial agents for empirical therapy of UTI. However, it is seen that Fluoroquinolone resistance among urinary isolates and its association with multi drug resistance, especially beta lactam antibiotics is on the rise. Hence, this study was undertaken to evaluate the prevalence of quinolone resistance and its co-existence with Extended Spectrum β-Lactamase (ESBL) production among urinary isolates of E.coli. Objectives: 1. To determine the prevalence of quinolone resistance among urinary isolates of E.coli. 2. To evaluate co-existence of quinolone resistance with ESBL production among them. Materials and Methods: All urine samples received at Microbiology laboratory, McGann hospital, attached to Shimoga Institute of Medical Sciences, Shimoga, was included for study. E.coli was isolated by following standard conventional microbiological methods. These isolates were subjected to antimicrobial susceptibility testing by Kirby-Bauer disk diffusion method and ESBL production was tested according to CLSI guidelines 2014. Results: In a total of 932 urine samples, 82 E.coli were isolated. Out of these 82 E.coli isolates, 62 (75.6%) isolates showed quinolone resistance. And out of these 62 quinolone resistant isolates, 54(88.5%) isolates showed ESBL production. Conclusion: Our study shows significant co-existence of quinolone resistance and ESBL production among the urinary isolates of E.coli. The result of this study justifies the need for continuous surveillance, monitoring and revision of the antibiotic use policies to control the dissemination of resistance determinants among the pathogen.
Authors and Affiliations
Arundathi HA, Mallikarjun Koppad, Halesh LH, Siddesh KC
Keywords
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