Study of bacterial microbiota in patients with acute exacerbation of bronchial asthma and chronic bronchitis

Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 3

Abstract

Introduction: Microbiota of respiratory tract may be responsible for acute exacerbation of bronchial asthma and chronic bronchitis. So the present study was carried out to find out specific bacterial etiological agents’ involvement in acute exacerbation of these diseases. Materials and Methods: Sputum samples from patients of bronchial asthma, chronic bronchitis during acute exacerbation and endotracheal aspirates from control cases were processed to isolate the microbiota of respiratory tract using quantitative methods after liquefying and diluting the samples. Significant and insignificant bacterial counts from the samples were determined. Grading of pus cells and bacterial morphology was seen by Gram's staining. Results: Pus cell grading of 1+ to 3+ were seen in the both cases of bronchial asthma and chronic bronchitis whereas pus cells were not found in control cases. Percentage of microorganisms by Gram's staining in asthmatics, chronic bronchitis, and controls cases were 93.33%, 86.67%, 76.67% respectively. Among the pathogenic bacteria, percentage of significant count of Staphylococcus aureus and Klebsiella pneumonia were found to be high in chronic bronchitis cases when compared these pathogens in asthmatics. In bronchial asthma cases, the percentage of significant count of Streptococcus pyogenes, Proteus mirablis and Pseudomonas aeruginosa were high as compared to chronic bronchitis cases. In chronic bronchitis patients and asthmatics, significant count of non-pathogens were 16.67% and 13.33% respectively. Conclusion: The quantitative cultures showing significant numbers as 104/ml and 107 /ml of pathogenic and nonpathogenic bacteria respectively are found to be associated with acute exacerbation of bronchial asthma and chronic bronchitis.

Authors and Affiliations

Aruna Solanki, Arun More, Jyoti Tomar

Keywords

Related Articles

Recent trend of bacterial aetiology of respiratory tract infections with special reference to Escherichia Coli

Background and Objective: Respiratory tract infections (RTIs) are among the most common infectious diseases affecting humans worldwide. Pathogenic isolates of E.coli have relatively high potentials for developing resista...

Mutagenesis and screening of Penicillium oxalicum (Dal 5) for enhancing cellulase production

In the modern era due to increasing fuel demand prices of crude oil is increasing day by days So the need for bioenergy sources is increasing in coming years Of all the biomass present in the world lignocelluloses as ind...

Review on importance of antimicrobial prophylaxis and sterilization procedures in general surgery

Antimicrobial agents are compounds which inhibit the growth, or kill microorganisms. Antimicrobial agents can be used for either treatment or prevention of infectious disease but must be used properly and accurately. The...

A case of mycotic keratitis caused by Colletotrichum, a plant pathogen

Mycotic keratitis has emerged as a major ophthalmic problem. Fungal keratitis is usually caused by Aspergillus, Fusarium, Curvularia and Candida species. But one should be aware of other rare environmental pathogens caus...

Speciation and antibiogram of Staphylococcus isolated in a tertiary care centre

Introduction: Staphylococcus have become common cause of skin and soft tissue infections. Resistance to a number of drugs have increased and methicillin resistant Staphylococcus aureus (MRSA) and inducible clindamycin re...

Download PDF file
  • EP ID EP472195
  • DOI 10.18231/2394-5478.2018.0086
  • Views 99
  • Downloads 0

How To Cite

Aruna Solanki, Arun More, Jyoti Tomar (2018). Study of bacterial microbiota in patients with acute exacerbation of bronchial asthma and chronic bronchitis. Indian Journal of Microbiology Research, 5(3), 415-420. https://europub.co.uk/articles/-A-472195