Combination of Gymnemasylvestre and Combretummicranthum Methanol Leaf ExtractsProduced Synergistic Hypoglycaemic Activity in Alloxan Diabetic Mice
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2017, Vol 3, Issue 11
Abstract
Abstract:Herbal combinations when used together may sometimes produce enhanced, same or diminished effect. As a result, herb combinations used in the management of diabetes mellitus need to be thoroughly investigated to provide the best effect in reducing the major underlying cause (hyperglycemia) of the disease. Aqueous extracts of Gymnemasylvestre (GS) and Combretummicranthum (CM) leaves were subjected to solvent-solvent partitioning along different polarities. The best fractions (methanol) in terms of in vivo fasting blood glucose (FBG) in alloxan-diabetic mice were subjected to column chromatography and preparative-TLC to obtain sub-fractions and sub-sub-fractions respectively. Sub-fractions D and G for GS and CM respectively gave the best reduction in FBG in vivo while sub-sub-fractions D4 and G4 yielded highest percentage reduction in FBG in alloxan-diabetic mice. The probable mode of action of the phenolic compounds identified by UHPLC-QTOF-MS/MS in both plants could be by inhibiting activities of α-amylase and α-glucosidase. At a dose of 10 mg/kg body weight, a 1:1 combination of GS-D4 and CM-G4 sub-sub-fractions, containing mainly phenolic compounds, significantly (P < 0.05) reduced FBG to 58.25±7.54% in 2 hours and 69.11±6.78% in 4 hours in a synergistic manner. Keywords:Herbal combination; synergy; diabetes mellitus
Authors and Affiliations
Ibrahim A Ibrahim A, Onyike E, Nok A. J, Umar I. A
Keywords
Related Articles
- EP ID EP393603
- DOI -
- Views 64
- Downloads 0
Research of Diuretic Action of 5-Substituted Derivatives of 1,3,4-Thiadiazole
Abstract: Search and creation new biologically active compounds which exhibit diuretic action is an current task of the modern Medical Chemistry. In order to identify diuretic activity a series of 5-substituted of 1,3,4-...
Nurses’ perception of medication administration errors in children hospital, Lahore, Pakistan
Abstract: Nurses provide care to patients and they feel hope to get comfort and recover. Nurses use the knowledge and skills to provide care to the patients. There are many health care professionals who involved in provi...
Mineral Content and Chemical Composition of Napier (Pennisetumpurpureum) Grass
Abstract:Napier grass is a popular tropical grass, employed by farmers in livestock nutrition. In this work, the matrices of matured shoots of P. purpureum (schumach) commonly called Napier grass, were subjected to pr...
The Role of the Pathologist in the Diagnosis of Metastasis of Unknown Primary Origin
Abstract:Metastasis of unknown primary origin (MUPO) account for 2–3 % of all malignancies in Western countries and represent a heterogeneous, often aggressive, and clinically challenging group of tumors with early metas...
Acute Interstitial Nephritis Associated Multiple Myeloma: A Case Report
Abstract:Patients with multiple myeloma may present a variety of renal manifestations as a result of damage from circulating light chain immunoglobulin components. The renal alterations can interest any of the renal comp...