COMPARATIVE ANALYSIS OF CARDIAC GLYCOSIDES AND QUANTIFICATION OF PERUVOSIDE FROM THEVETIA NERIIFOLIA, JUSS FRUIT RIND EXTRACTS THROUGH HPTLC FINGERPRINTING
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 8
Abstract
Objective: Present investigation was carried out to determine the cardiac glycoside profile and to quantify the powerful cardiotonic drug peruvoside in the fruit wall of Thevetia neriifolia, Juss a medicinal plant used in indigenous system of medicine since decades.Methods: Shade dried powdered samples were subjected to soxhlet extraction using different solvents, and various forms of cardiac glycosides were analysed in all fractions using TLC plates. Positive samples for peruvoside were subjected to HPTLC fingerprinting on silica gel 60 F254 plates using chloroform: methanol (8:2v/v) mixture as mobile phase.Results: Fingerprint profile showed the presence of 6-14 cardiac glycosides in all three morphovariat plants. Densitometric analysis at 220 nm showed a peak at Rf 0.67±0.01 to the reference drug peruvoside, and in all samples analysed an identical compound corresponding to peruvoside was eluted within a range of 0.63 to 0.75 with minor variations. It was noticed that peruvoside was the third largest cardiac glycoside extracted from the fruit rind using chloroform.Conclusion: In all studied morphoforms, about 3.25% peruvoside was detected in the rind which was the neglected and unused portion of fruit. Using fingerprinting technique, all cardiac glycosides can be effectively separated and utilized for pharmaceutical purposes.
Authors and Affiliations
Nesy Ibrahim, Lizzy Mathew
Keywords
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