Comparative analysis of genetic structure of a micro-population of gray Ukrainian breed cattle by DNA markers
Journal Title: Біологія тварин - Year 2016, Vol 18, Issue 1
Abstract
The use of advances in genetics in cattle breeding from identification to parentage determination significantly improves its efficiency. The genotyping of animals by means of microsatellite loci is mandatory for recognition of animals as breeding. The aim of our study was the analysis of genetic resources of cattle of Gray Ukrainian breed by means of microsatellite loci of DNA. To achieve this aim we used 2 microsatellite loci — BM2113 and TGLA 227. The material for the research was cattle of Gray Ukrainian breed from two households — “Golosiyevo” (Brovary district, Kyiv region) and “Voronkiv” (Boryspil district, Kyiv region). Genetic analysis of cattle belonging to “Voronkiv” was conducted in the Ukrainian Laboratory of Quality and Safety of Agricultural Products. Genetic analysis of cattle belonging to “Golosiyevo” was conducted in the State scientific institution “All-Russian Research Institute of Livestock of Russian Academy of Agricultural Sciences”. As a result of conducted studies of two herds of cattle sufficiently high level of polymorphism was identified. In cattle belonging “Voronkiv” higher level of polymorphism with locus TGLA227 was observed. All studied animals of this household with locus TGLA227 were heterozygotes. It showed the value of observed heterozygosity. The deficiency of heterozygous genotypes (2,5 %) was identified in the herd of “Golosiyevo”. On the contrary, in cattle belonging “Voronkiv” there was an excess of heterozygous genotypes (17,7 %). The future genetic analysis of cattle of Gray Ukrainian breed by means of more microsatellite loci is perspective.
Authors and Affiliations
Y. Huzeyev, O. Melnyk, V. Spyrydonov, S. Melnychuk
Keywords
Related Articles
- EP ID EP234452
- DOI -
- Views 136
- Downloads 0
Content of lipids in tissues of the melliferous bees additional fed with soybean meal, sugar syrup and citrates of Co and Ni
The article presents the research results of the influence of addition to melliferous bees sugar syrup (SS), SS and soybean flour (SF) (II) and SS, SF and Co and Ni citrates (СoNC + NiNC) in a dose of 2 and 1 mg separate...
Assessment of immunotoxic effects of desloratadine under the experimental conditions in laboratory animals
Research methods of immunotoxic effect of pollutants are informative even when other tests are unable to detect adverse effects of toxicant. The effect of desloratadine (third-generation antihistamine) on the immune syst...
Activity of periplasmic hydrogenase of the intestinal sulfate-reducing bacteria
In this paper we present a periplasmic hydrogenase activity of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine. The effect of temperature and...
Features of microbiocenosis of the poultry organism with the mixed pasteurellosis and ascaridosis disease
The purpose was to study the quantitative composition of microorganisms at the mixed pasteurellosis and ascaridosis disease of poultry. The article presents the results of microbiological monitoring and quantitative comp...
Comparative developmental dynamic of hens and quails caecums microbocenosis
The implementation of high poultry performance has directly related to animal health, such as the formation of stable intestine microbocenosis. The preparation for correction the intestinal microfloras composition is app...