COMPARATIVE ANALYSIS OF MORPHOLOGY AND PHYTOCHEMICAL CONSTITUENTS IN DIFFERENT POPULATIONS AND MORPHOTYPES OF DATURA INNOXIA MILL. AND DATURA METEL L. FROM PUNJAB PLAINS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.
Authors and Affiliations
Mahinder Partap, Raghbir Chand Gupta, Saroj Kumar Pradhan
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