COMPARATIVE ANALYSIS OF THE EFICENCY OF POWERPLEX 16 AND POWERPLEX FUSION MULTIPLEX STR KITS IN THE ANALYSIS OF THE CHALLENGING FORENSIC SAMPLES
Journal Title: Genetics & Applications - Year 2017, Vol 1, Issue 2
Abstract
Since the introduction of the term low copy number DNA, also referred as low template DNA, touch DNA or trace DNA analysis, it has quickly become focal point of forensic DNA testing as well as other DNA based studies. Low template DNA (ltDNA) samples can be described as the samples which involve single source samples with template DNA in concentrations below 100 picograms (pg). Due to sensitivity of ltDNA samples to contamination, it is of great importance to optimize performance of the multiplex STR systems and existing protocols to increase chance of successful analysis. The main objective of this study was analysis of 20 challenging samples (skeletal remains, cigarette buts, chewing gum, poorly collected buccal swabs etc.) mostly low template DNA samples, preliminarily profiled by PowerPlex® 16 multiplex STR systems and additionally processed with new generation multiplex STR kit PowerPlex® Fusion. Sample isolation was done using a standard phenol-chloroform method for bone samples and DNeasy® Blood and Tissue Kit for other forensic samples. PowerPlex® 16 (PP16), multiplex STR system and PowerPlex® Fusion (PP Fusion) were used for co-amplification of 15 and 24 autosomal STR loci respectively. Results of this preliminary study suggest that PP Fusion primer set is better optimized for the analysis of ltDNA samples, and it is more robust regarding presence of the potential PCR inhibitors.
Authors and Affiliations
Zlatan Husukić, Amela Pilav, Jasmina Čakar, Mirela Džehverović, Serkan Dogan, Damir Marjanović
Keywords
Related Articles
- EP ID EP349747
- DOI 10.31383/ga.vol1iss2pp50-55
- Views 114
- Downloads 0
Intermediate frequency magnetic field at 250.8 kHz does not induce DNA damage or “Adaptive Response” in vitro
The aim of this study was to examine whether intermediate frequency (IF) magnetic field (MF) induces genotoxic effects or play a role in the induction of adaptive response after combined exposure to IF MF and ionizing ra...
COMPARISON OF TWO DIFFERENT MULTIPLEX SYSTEMS IN CALCULATING KINSHIP AMONG CLOSE RELATIVES
This study compares the results obtained using two multiplex systems, PowerPlex® 16 System and PowerPlex® Fusion System, to evaluate the probability of a specific kinship relationship between the offspring of three pairs...
MUTATION ANALYSIS OF AUTOSOMAL STR LOCI COMMONLY USED IN PATERNITY TESTING IN BOSNIA AND HERZEGOVINA
Mutation analysis in forensic genetics and occurrence of mutations at short-tandem repeat (STR) loci, are very important in paternity testing and precise elucidation of obtained genetic profiles. To determine these locus...
INFLUENCE OF BASIC VARIABLES ON MICRONUCLEI FREQUENCY AND CHROMOSOMAL ABERRATIONS IN GENERAL POPULATION OF FB&H
The aim of this study was to determine the values of micronuclei (MN) and structural chromosomal aberrations (CA) in peripheral blood lymphocytes from 200 healthy participants of both genders from general population of F...
ALRATIO - R script for the analysis of relation between the effective and the detected number of alleles
/