COMPARATIVE ANALYSIS OF THE GROWTH OF Helicobacter Pylori IN DIFFERENT BLOOD AGAR MEDIUM
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2017, Vol 6, Issue 5
Abstract
Objective: Helicobacter pylori are the gram negative pathogenic bacteria mainly associated with gastrointestinal disorders. The growth of the H. pylori has been achieved only by inoculating the clinical specimen and storage in blood agar only. The objective of this research was to compare the growth of H. pylori in different blood agar medium to elucidate whether the medium provided considerable growth of H. pylori in vitro. Methods: H. pylori was inoculated in human, goat, sheep, fish and chicken blood agar plates and incubated in anaerobic under microaerophilic (5% O2, 10% CO2 and 85% N2) conditions at 37°C for 4 days. After successful incubation, significant differences in growth was observed and further confirmed based on colony morphology, Gram staining and biochemical tests. Results: In human blood agar plates, it was observed that creditable colony size and growth of H. pylori. In goat blood also showed more or less equal results same like human blood; whereas minimal growth was observed in chicken, and no growth was observed in sheep and fish blood agar plates. Even though the H. pylori colonies were observed on chicken blood agar plates, the urease test of the colonies are very slow reactive than human and goat blood agar. But other parameters and characterization of goat blood agar are more or less equal like human blood. Conclusion: No previous report observed in the determination of H. pylori growth in different blood culture medium. Thus, this study is suggesting the usage of goat blood as the alternative for the human blood agar for H. pylori isolation and subculturing.
Authors and Affiliations
Sampath Gattu, N. Prabhusaran, k Sridharan, N Kayalvizhi
Keywords
Related Articles
- EP ID EP436124
- DOI -
- Views 122
- Downloads 0
RP-HPLC METHOD FOR DETERMINING THE LEVELS OF GUAIPHENESIN AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORMS
A simple, precise and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of guaiphenesin and phenylephrine in their tablet dosage form. The chromatographic...
TO DESIGN MICROSPHERES OF THE ANTIBACTERIAL DRUG CEFACLOR AND OPTIMIZE ITS CHARACTERISTICS
Aim: This study aims to design microspheres of the antibacterial drug Cefaclor and optimize its characteristics to improve the bioavailability as well as to reduce its side effects. This utilizes statistical software for...
EVALUATION OF THE EFFECT OF BENZENE-CONTAMINATED WATER ON THE GRAVIMETRY, SERUM CHOLESTEROL AND TRIGLYCERIDE CONCENTRATIONS OF AFRICAN CATFISH (CLARIAS GARIEPINUS)
Objective: The biochemistry of Clarias gariepinus cultivated in benzene-contaminated water for a period of 8 weeks was studied. Methods: Twenty African catfish were divided into two groups of ten fish each and cultivated...
MOLECULAR INTERACTION OF NATURAL BIFLAVONOIDS, AS COMPETITIVE ANTAGONISTS, WITH AROMATASE RELATED TO BREAST CANCER: AN IN SILICO APPROACH
Objective: Understanding the mechanism of inhibition exerted on aromatase by natural compounds is essential since such compounds may be helpful as a source of efficacious therapeutic agents for the management of postmeno...
COMPREHENSIVE NURSING CARE: CONCEPT ANALYSIS WITH THE EVOLUTIONARY APPROACH
Field and Target: Comprehensive nursing care has always been one of the main arguments of nursing profession. One simple definition of Holism is that whole of one thing is more than the sum of its components. The aim of...