RP-HPLC METHOD FOR DETERMINING THE LEVELS OF GUAIPHENESIN AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2016, Vol 5, Issue 7
Abstract
A simple, precise and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of guaiphenesin and phenylephrine in their tablet dosage form. The chromatographic conditions were standardized using an Inertsil column,C18 (150x4.6 ID) 5µm with UV detection at 277nm , and the mobile phase consisted of sodium dihydrogen phosphate buffer: Acetonitrile (30:70).The retention times of guaiphenesin and phenylephrine were About 3.497 min for Guaifenesin and 2.353min for Phenylephrine, respectively. The calibration curves were linear with correlation coefficients of 0.9987, 0.9988, 0.9981 and 0.9981 over a concentration range of 4.0–24.0 µg/ml for guaiphenesin, 5.0–30.0 µg/ml for phenylephrine, respectively. The proposed method has been validated according to the ICH guidelines and was successfully applied to estimate the levels of two drugs in a combined formulation with good accuracy and precision.
Authors and Affiliations
malladi babu, pavan kumar chadalawada, s. manohar babu
SIGNIFICANT ROLE OF SOXHLET EXTRACTION PROCESS IN PHYTOCHEMICAL RESEARCH
Soxhlet extraction is also known as the hot continuous extraction process the main advantage of this method is complete extraction in minimum amount of solvent. Aim and Objectives: This review is focused on various pre e...
A REVIEW ON CLINICAL TRIALS: WHY TO INTRODUCE ZERO PHASE
Objective: The main objective of this study is to know clinical trials in nutshell and phase 0 clinical trial are to establish at the very earliest opportunity-before large numbers of patients have been accrued and expos...
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM PROSOPIS CINERARIA
Objective: Prosopis cineraria is a tree of remarkable importance and a rich source of secondary metabolites. The present study was carried out to investigate the flavonoid content present in the leav...
DESIGNING AN INTERACTIONAL MODEL BETWEEN INSURER INSTITUTES AND GOVERNMENTAL INSTITUTES FOR IMPROVING TREATMENT SERVICES IN YASOUJ CITY 2017
This study was conducted with the aim of designing an interactional model between insurer institutes and governmental hospitals for improvement of treatment services of Yasouj City in 2017. This study has been performed...
LIMITED BUT IMMERGING: INFORMATION TECHNOLOGY IN PHARMACY
Objective: In contemporary years, the quantity of medicine and drug treatments has larger extremely that are quite often more strong and extra selective and in addition, the literature relating to medicines has extended...