RP-HPLC METHOD FOR DETERMINING THE LEVELS OF GUAIPHENESIN AND PHENYLEPHRINE IN PHARMACEUTICAL DOSAGE FORMS
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2016, Vol 5, Issue 7
Abstract
A simple, precise and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of guaiphenesin and phenylephrine in their tablet dosage form. The chromatographic conditions were standardized using an Inertsil column,C18 (150x4.6 ID) 5µm with UV detection at 277nm , and the mobile phase consisted of sodium dihydrogen phosphate buffer: Acetonitrile (30:70).The retention times of guaiphenesin and phenylephrine were About 3.497 min for Guaifenesin and 2.353min for Phenylephrine, respectively. The calibration curves were linear with correlation coefficients of 0.9987, 0.9988, 0.9981 and 0.9981 over a concentration range of 4.0–24.0 µg/ml for guaiphenesin, 5.0–30.0 µg/ml for phenylephrine, respectively. The proposed method has been validated according to the ICH guidelines and was successfully applied to estimate the levels of two drugs in a combined formulation with good accuracy and precision.
Authors and Affiliations
malladi babu, pavan kumar chadalawada, s. manohar babu
Keywords
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