Comparative evaluation of five different storage media and temperature effect on human periodontal ligament fibroblast viability
Journal Title: Caspian Journal of Dental Research - Year 2019, Vol 8, Issue 1
Abstract
Introduction: Following dental avulsion, the immediate recommended treatment is tooth replantation to avoid adverse effects. Therefore, the tooth must be stored in a physiological storage medium to preserve the viability of the periodontal ligament fibroblast (PDLF) cells during transportation to dental office. The aim of this study was to determine the effect of several storage media in preserving the viability of human PDLF cells at different times and temperatures. Materials & Methods: In this experimental study, the human PDL cells were obtained from the healthy extracted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). The studied media were DMEM (10% FBS + 1% penicillin G Na (10000 IU) + 1% streptomycin (10 mg)), tap water, sterilized whole milk, zero fat milk and soy milk. After the cells had reached sufficient density in the plate, they were added to the experimental media and kept at 1, 2, 4 and 24 hours at 4° and 37oCentigrade. After incubation, the cell viability was determined by tetrazolium salt-based colorimetric (MTT) assay. The results were statistically analyzed using Kruskal–Wallis and post hoc Tests. Results: Whole milk and DMEM showed significantly higher protective effect than other media. The viability of PDL cells had significant difference at 4oC compared to 37oC at 4 and 24 h in DMEM group and at 24 h in whole milk group (p≤0.05). Conclusion: The results have suggested that the whole milk like DMEM have enough essential nutrients for PDLFs and have confirmed the hypothesis that the milk similar to HBSS or DMEM might be effective in preserving the PDLF cells.
Authors and Affiliations
Zahra Sadat Madani, Zinat Sadat Rezaie, Ebrahim Zabihi, Zeinab Abedian
Keywords
Related Articles
- EP ID EP581525
- DOI 10.22088/cjdr.8.1.8
- Views 97
- Downloads 0
Histopathological evaluation of dental pulp of cat after using of sodium sulfonate in composite restorative treatment
Introduction: Previous researches showed that neutral soaps significantly increased bond strength and decreased microleakage of dentin bonding agents. The aim of this study was histopathological evaluation of the effect...
The effect of periodontal therapy on IL-17 and IL-23 in Gingival Crevicular Fluid (GCF) of patients with severe periodontitis
Introduction: Cytokines are the important factors in the progression of periodontal diseases. The aim of this study was to find out the effect of the first phase of the periodontal therapy on the amount of existing IL-17...
Dimensional changes of dental arch following non-extraction orthodontic treatment
Introduction: Since the emergence of normal occlusion concept, selecting an appropriate treatment plan with or without tooth extraction has been a controversial subject. Nowadays, both methods can be successfully used fo...
Plexiform schwannoma of the floor of the mouth: a case report
Plexiform schwannoma is a type of schwannoma that can be occured at any age and any site of the oral cavity. In the gross and histopathological features, it shows plexiform or multinodular growth pattern. This variant of...
Frequency of oral cancer in Babol during 2012-2016
Introduction: Regarding the lack of data on the frequency of oral cancer in Babol, the aim of this study was to evaluate the frequency of oral cancer in Babol during 2012-2016. Material & Methods: This retrospective cro...