Comparative evaluation of five different storage media and temperature effect on human periodontal ligament fibroblast viability
Journal Title: Caspian Journal of Dental Research - Year 2019, Vol 8, Issue 1
Abstract
Introduction: Following dental avulsion, the immediate recommended treatment is tooth replantation to avoid adverse effects. Therefore, the tooth must be stored in a physiological storage medium to preserve the viability of the periodontal ligament fibroblast (PDLF) cells during transportation to dental office. The aim of this study was to determine the effect of several storage media in preserving the viability of human PDLF cells at different times and temperatures. Materials & Methods: In this experimental study, the human PDL cells were obtained from the healthy extracted third molars and cultured in Dulbecco's Modified Eagle Medium (DMEM). The studied media were DMEM (10% FBS + 1% penicillin G Na (10000 IU) + 1% streptomycin (10 mg)), tap water, sterilized whole milk, zero fat milk and soy milk. After the cells had reached sufficient density in the plate, they were added to the experimental media and kept at 1, 2, 4 and 24 hours at 4° and 37oCentigrade. After incubation, the cell viability was determined by tetrazolium salt-based colorimetric (MTT) assay. The results were statistically analyzed using Kruskal–Wallis and post hoc Tests. Results: Whole milk and DMEM showed significantly higher protective effect than other media. The viability of PDL cells had significant difference at 4oC compared to 37oC at 4 and 24 h in DMEM group and at 24 h in whole milk group (p≤0.05). Conclusion: The results have suggested that the whole milk like DMEM have enough essential nutrients for PDLFs and have confirmed the hypothesis that the milk similar to HBSS or DMEM might be effective in preserving the PDLF cells.
Authors and Affiliations
Zahra Sadat Madani, Zinat Sadat Rezaie, Ebrahim Zabihi, Zeinab Abedian
Keywords
Related Articles
- EP ID EP581525
- DOI 10.22088/cjdr.8.1.8
- Views 91
- Downloads 0
Gingival microleakage in class II composite restorations using different flowable composites as liner: an in vitro evaluation
Introduction: One of the main disadvantages of composites is marginal microleakage; using flowable composites as a liner beneath composite restorations has been recommended to reduce microleakage. The aim of this study w...
A comparative study of the effects of QTH and LED light curing units on the surface hardness of colored compomer and Hybrid composites
Introduction: One factor affecting the degree of polymerization is the type of light-curing device. The aim of this study was to compare the effects of LED and QTH light curing units on the surface hardness of composite...
Evaluation of the accuracy of two apex locators in endodontic treatment and retreatments: an Ex vivo study
Introduction: Working length determination is important in successful endodontic treatment and retreatment. This study evaluated the accuracy of two electronic apex locators Root ZX and Raypex®6 (EALs) in determining the...
Knowledge and practices concerning the effects of ionizing radiation and x-ray protection methods in dental offices in Babol, 2013
Introduction: As the absorption of radiation in environment is increasing, the present study was performed to evaluate the knowledge and practices of Babol’s dentists about x-ray protection. Materials &Methods: This cros...
Evaluation of pretreatment with gelofen and novafen on pain relief after endodontic treatment; A double-blind randomized clinical trial study
Introduction: Feeling pain after root canal therapy has always been a major problem for patients and dentists. One of the suggested methods to manage the pain is using the prophylactic medication before treatment process...