Comparative Investigation of In Vitro Genotoxicity of ProMutagens Using Human-Derived Hepatoma HepG2 Cells with Human and Rat Liver S9
Journal Title: Advances in Clinical Toxicology - Year 2024, Vol 9, Issue 3
Abstract
The genotoxicity of pro-mutagens has been traditionally detected by test procedures with metabolically incompetent cells requiring addition of exogenous activation mixtures, such as rat liver S9. HepG2 cells established from huma hepatoma in 1979 have metabolic activation ability. For this characteristic, HepG2 cells were tried to be used in the genotoxicity test. Since metabolic activation ability in HepG2 cells is not the same as that in exogenous activation mixtures, it is necessary to ascertain whether HepG2 cells could be used to detect the genotoxicity of various kinds of pro-mutagens and to compare the detecting ability between HepG2 cells and metabolically incompetent cells under exogenous activation mixtures. The ability of HepG2 cells to detect the genotoxicity of 38 pro-mutagens was studied to compare that of metabolically incompetent human lymphoblastoid WTK1 cells under human and rat S9’s. Present results showed that the ability of HepG2 cells to detect the genotoxicity of pro-mutagens parallels qualitatively to that of WTK1 cells under human liver S9 and cytochrome (CYP) induced rat liver S9 even if the existence of species difference in CYP isoforms. Therefore, HepG2 cells could be usefully used to detect the gentoxicity of pro-mutagens for the purpose of the evaluation of the genotoxic risk for humans.
Authors and Affiliations
Kawaguchi S , Okutani S , Yamamoto A , Nakamura T and Sasaki Yu F
Keywords
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- EP ID EP754568
- DOI 10.23880/act-16000314
- Views 11
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