Comparative performance of three sampling techniques to detect airborne [i]Salmonella[/i] species in poultry farms
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2014, Vol 21, Issue 1
Abstract
Sampling techniques to detect airborne [i]Salmonella[/i] species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of [i]Salmonella[/i] enteritidis. The rearing cycle lasted 42 days during the summer. Airborne [i]Salmonella[/i] spp. were sampled weekly using impaction, gravitational settling, and impingement techniques. Additionally, [i]Salmonella[/i] spp. were sampled on feeders, drinkers, walls, and in the litter. Environmental conditions (temperature, relative humidity, and airborne particulate matter (PM) concentration) were monitored during the rearing cycle. The presence of [i]Salmonella[/i] spp. was determined by culture-dependent and molecular methods. No cultivable [i]Salmonella[/i] spp. were recovered from the poultry houses’ surfaces, the litter, or the air before inoculation. After inoculation, cultivable [i]Salmonella[/i] spp. were recovered from the surfaces and in the litter. Airborne cultivable [i]Salmonella[/i] spp. Were detected using impaction and gravitational settling one or two weeks after the detection of [i]Salmonella[/i] spp. in the litter. No cultivable [i]Salmonella[/i] spp. were recovered using impingement based on culture-dependent techniques. At low airborne concentrations, the use of impingement for the quantification or detection of cultivable airborne Salmonella spp. is not recommended. In these cases, a combination of culture-dependent and culture-independent methods is recommended. These data are valuable to improve current measures to control the transmission of pathogens in livestock environments and for optimising the sampling and detection of airborne[i] Salmonella[/i] spp. in practical conditions.
Authors and Affiliations
Elisa Adell, Verónica Moset, Yang Zhao, Ana Jiménez-Belenguer, Alba Cerisuelo, María Cambra-López
Keywords
Related Articles
- EP ID EP80730
- DOI -
- Views 123
- Downloads 0
Etiopathogenesis of midfacial fractures in patients treated at Maxillofacial Surgery Clinic , Medical University of Lublin in 2007–2011
[b]Introduction:[/b] With the growing industrialization and the increasingly rapid pace of life the number and variety of injuries are growing higher. In the midst of injuries, the midfacial injuries constitute a conside...
Influence of calcium intake on bone mineral density and incidence of fractures in treatmentnaive women from Lodz urban area – a part of EPOLOS study
Introduction. Inadequate calcium intake is a recognized osteoporosis risk factor. The aim of the study was to estimate calcium intake in women in the Łódż population, the influence of calcium intake on bone mineral densi...
Ammonia, dust and bacteria in welfare-oriented systems for laying hens.
The use of litter and manure in welfare-oriented systems for laying hens may negatively affect the air quality and the work environment. The objective of the current case study was to compare concentrations of ammonia, d...
Visual assessment of voice disorders in patients with occupational dysphonia
[b]introduction[/b]. In a group of persons using the voice occupationally, the frequent symptoms are hoarseness, voice fatigability and aphonia. Pathological changes in the larynx may have organic or functional character...
Comparative performance of three sampling techniques to detect airborne [i]Salmonella[/i] species in poultry farms
Sampling techniques to detect airborne [i]Salmonella[/i] species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of [i]Salmonella[/i] enteritidis...