COMPARATIVE STUDIES OF In vitro AND In vivo RAISED SEEDLINGS OF Oryza sativaL
Journal Title: Indian Journal of Scientific Research - Year 2015, Vol 5, Issue 2
Abstract
Seeds of three Indian rice (Oryza sativa L.) varieties Sita, Rupali and Swarna Masuri were evaluated for in vitro (using MS media supplemented with 0.5mg/l IAA) and in vivo (using water soaked cotton) germination. Comparison of those germinated seedlings on the basis of in vivo and in vitro condition by using morphological, biochemical and analytical parameter were done. Seeds were used as explant source on both the culture condition. In morphological study, analysing growth rate i.e the length of geminating seedlings, best response were observed for in vitro ones. However, both the culture conditions were not found to be differentiate in the viability frequency of varities and they resulted more or less equal. In contrast to biochemical study, the in vitro varieties were seen to be rich in chlorophyll and starch content whereas the in vivo were higher in total phenolic content and more potential in catalase and peroxidase enzyme activity. The ability of accumulation of protein was imprecisely same for all three varities that grown up on different culture condition. Spectrophotometric estimation of starch and total phenolic were became more clarified when they showed same supportive result in analytical study of thin layer chromatography. Native gel electrophoresis was performed for esterase isozyme by result which only differ in their concentration but not in any bands.
Authors and Affiliations
AMITAVA ROY
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