Comparative Study between Greeks and Albanians with the Use of Restriction Fragment Length Polymorphisms of Apolipoprotein B Gene
Journal Title: Annual Research & Review in Biology - Year 2015, Vol 7, Issue 2
Abstract
Aim: The aim of this study was to compare the genetic polymorphisms of apolipoprotein B gene between two different populations, Greeks and Albanians living in Greece and to investigate the possibility of discriminating the two populations by using these polymorphisms. Methodology: Restriction fragment length polymorphisms at codons 2488 (XbaI) and 4154 (EcoRI) of the apolipoprotein B gene were investigated in the above populations, in order to determine if there are differences between them. Two specific DNA regions, each containing the polymorphic site, were amplified by polymerase chain reaction. The products were digested and electrophoresis on 2% agarose gel was followed. A total number of 160 unrelated individuals from each population were randomly collected. Results: The allelic frequencies of the samples from Greeks and Albanians showed variability patterns for the XbaI and EcoRI Restriction Fragment Length Polymorphisms. For Greeks and Albanians the presence of E+/+ genotype was almost the same (67.5% and 70.6% respectively), without statistical significant differences and the E-/- genotype showed low common presence (6.3% and 2.5% respectively). The presence of X-/- genotype had almost the same ratio for the two populations (48.1% for Greeks and 39.4% for Albanians) and the presence of X+/+ genotype was low enough for both of them. Conclusion: The study of the two populations (Greeks and Albanians) did not show any statistically significant differences concerning the frequency of the genotypes of XbaI and EcoRI polymorphisms of the APOB gene.
Authors and Affiliations
Alexandra Ampati, Stauroula Papadodima, Eleni Zorba, Chara Spiliopoulou, Maria Georgiou
Keywords
Related Articles
- EP ID EP353381
- DOI 10.9734/ARRB/2015/16246
- Views 103
- Downloads 0
First Report on Colletotrichum acutatum of Olives in Morocco
Anthracnose is an important disease affecting the yield and quality of olive production (olives, olive oil). Symptoms of anthracnose, circular soft decayed spots were observed on olives in the region of Ouazzane (North o...
Semen Characteristics of Rabbit Bucks Orally Administered Exogenous L-Selenomethionine
An experiment was carried out to evaluate the semen quality of rabbits administered varied levels of exogenous L-Selenomethionine (L-SeMet). Twenty four male rabbits (10 months old) were randomly allotted to four treatme...
Reducing the Histopathological and Biochemical Toxicity of Aflatoxins Contaminated Soybean Using Ozone Treatment
Aims: Soybean which was more frequently used in Egypt for edible oil production and meal for animal feeding was chosen as oil seed. The study targeted to reduce the aflatoxins toxicity, improving safety using natural tre...
Purification of Paraoxonase (PON) from Sun Flower (Helianthus annuus) and Effects of Some Chemicals on Paraoxonase Activity In vitro
Aims: In this study, paraoxonase (PON) enzyme was purified from mature seeds of sun flower by using affinity chromatography (Sepharose-4B-L-tyrosine-1-naphthylamine) and the effects of some chemicals were tested on parao...
Control of Glossina spp (Diptera: Glossinidae) by Pathogenic Organisms and their by Products
Tsetse flies Glossina spp (Diptera: Glossinidae) are the dominant vectors of trypanosomes, causative organisms of human African Trypanosomiasis (HAT) and Animal African Trypanosomiasis (AAT) on the African continent. Pri...