Comparative Study of DPPH, ABTS and FRAP Assays for Determination of Antioxidant Activity
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 6
Abstract
Three simple spectrophotometric methods: (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging activity assay (DPPH), [2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)] free radical scavenging activity assay (ABTS) and Ferric ion Reducing Antioxidant Power assay (FRAP) for determination of antioxidant activity were compared. The antioxidant activity of aqueous extracts of the mycelia of three mushroom species (Ganoderma lucidum, Lentinus edodes and Agaricus bisporus) were compared for this study. A comparative account was made between the three antioxidant assays and their advantages and disadvantages were briefly reported along with the scope of application of each method. DPPH was found to be the most preferred method for the determination of antioxidant activity of mycelia of the three mushroom species because it could be rapidly performed and showed high reproducibility. Results were reported so that it becomes easy to select the right method that can give accurate results for the antioxidants in study.
Authors and Affiliations
Pooja Shah, H. A. Modi
Keywords
Related Articles
- EP ID EP20980
- DOI -
- Views 253
- Downloads 12
Design of Transmission System for Mini-Train
The mini train rides are commonly seen rides in amusement parks. The study is based on a mini train operating at Vismaya amusement park, Kannur. During its operation, the mini train had frequent problems related to powe...
Image De-Blurring and De-Noising using Weiner Filter and Anisotropic Diffusion for Natural Images
The proposed method basically represents how to de-blur and de-noise images using a wiener filter and Anisotropic Diffusion Filter. Basically wiener filter is used to produce an estimate of a desired image or target a r...
A Load Balancing Model for Job Scheduling using Graph Theory and Allocation of Time
The limited number of resource and unlimited number of user in cloud environment creates the issue of load balancing. The overload number of job in cloud creates the situation of cloud resource overloading. In cloud res...
Dynamic Routing with Security using a Blow fish algorithm in the Network System
In this paper is about encryption and decryption of the text, image, audio, video using a secret key block cipher which is for the dynamic routing with security using a cryptographic algorithm in the network system. The...
Implementation of IEEE 802.15.4 PHY Using Matlab
IEEE802.15.4 is a standard which specifies the physical and medium access control for low rate, low-cost, lowpower Wireless Personal Area Networks (LR-WPANs).This paper aim to develop a mat lab model for IEEE 802.15.4 p...