Comparative Study of Four Platelet-Rich Plasma Methods for Preparing Platelet Concentrates
Journal Title: Iranian Journal of Blood and Cancer - Year 2014, Vol 6, Issue 3
Abstract
Background: Platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. Platelet concentrates can be prepared by the apheresis method or from the whole blood using the Buffy-coat or Platelet-rich plasma methods. The purpose of this study was to compare four variations of platelet-rich plasma method based on double centrifugation protocol to identify the optimal centrifugation conditions with greatest platelet recovery and highest enrichment capacity for preparing platelet concentrates. Materials and Methods: Blood samples were obtained from 145 donors, chosen randomly from the donation department at the Tehran Blood Transfusion Center, Tehran, Iran. Four variations of platelet-rich plasma methods were selected for preparation of platelet concentrates. Platelet counting analysis was performed on samples and platelet enrichment and platelet recovery were calculated by investigating the correlation between the number of platelets in the whole blood, platelet-rich plasma and platelet concentrates. Results: Platelet count analysis revealed that the method performed with 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step had the highest platelet enrichment (5.59fold) and greatest platelet recovery (78.63%). Conclusion: Within the limits of this study, it can be concluded that 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step yielded the greatest platelet recovery and highest enrichment capacity and is a good choice for preparing platelet concentrates. Keywords: Platelet, concentrates, plasma, centrifugation, recovery, enrichment.
Authors and Affiliations
Mojgan Pourmokhtar, Ebadollah Salek Moghaddam, Fatemeh Abbasi, Nasrin Zarei
Keywords
Related Articles
- EP ID EP364635
- DOI -
- Views 71
- Downloads 0
Neuroendocrine Carcinoma of the Stomach A Case Report
Gastric neuroendocrine carcinoma is a rare tumor which has a poor prognosis. Herein, we present a 55-year-old woman who presented with complaints of recurrent vomiting, hematemesis and weight loss. Endoscopic examination...
Minor Histocompatibility Antigen HA-1 Frequency and Acute GVHD in Some Hematopoietic Stem Sell Transplanted Iranian Patients
Background: It is suggested that HA-1 mismatching among hematopoietic stem cell transplanted recipients-donors is associated with acute graft-versus-host disease (aGVHD). So the aim of this study was to evaluate HA-1 fre...
Stem Cell Therapy and Research Status in Iran : at A glance
This article has no abstract.
Patterns of Gelatinase-B Expression in Leukemic Cell Lines
Background: Gelatinase-B named MMP-9 (matrix metalloproteinase-9), is a protease that degrades collagen type ІV and V of extracellular matrix. MMP-9 production is increased in various types of cancers including leukemia...
Clinical Variables among Adult Patients with Chronic Idiopathic Thrombocytopenic Purpura in West Iran
Background: There are two forms of ITP: acute and chronic. The chronic form is defined by persistent thrombocytopenia for more than 12 months and mostly occurs among young women. The aim of the present study was to evalu...