COMPARATIVE STUDY OF LONG TERM PRESERVATION OF HUMAN CADAVERIC TISSUE
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 5
Abstract
Objectives: To study the effect of long term preservation of the gross and microscopic features of cadaveric tissue using different embalming techniques. Methodology: Eight human cadavers were randomly received and embalmed in Anatomy Laboratory. Cadavers were divided into four groups. In experiment I, cadavers were embalmed with traditional gravity fed embalming technique, without pre injection, using 5 lit fluid consisting of formaldehyde, phenol, water, spirit and glycerin amounting 1 litre each. In experiment II and III cadavers were embalmed using modern electric embalming machine to introduce same embalming fluid without and with the use of pre-injection respectively. In experiment IV cadavers were embalmed using high spirit, reduced formalin and low phenol combination along with pre-injection. Gross and microscopic examination was performed at the beginning and end of 2 years period for histological examination. Results: Preservation of the gross and microscopic features of human tissues was better in experiment III in which arterial injection was given with mechanical pump method along with pre-injection. In gravity fed method, hardening, browning and fragility was maximum and histological structure of skeletal muscle and neurovascular structures was of poor quality. Conclusion: The long term preservation of human cadaveric tissue at gross and microscopic level for academic purposes can be achieved with the use of arterial injection through mechanical pump along with pre-injection using 5-7 l of embalming fluid. Increasing water or spirit content improves resilience, decrease hardening but promotes fungal growth.
Authors and Affiliations
Dr. Alia Amin
Keywords
Related Articles
- EP ID EP617618
- DOI -
- Views 196
- Downloads 0
OPTIMUM CONDITIONS FOR CYTOSINE DEAMINASE PRODUCTION BY LOCAL ISOLATE OF ESCHERICHIA COLI E9.
This study was aimed to isolate a higher cytosine deaminase producer Escherichia coli and studying the optimum condition for its production, a total of twenty-five urine samples were collected for the isolation of E. col...
EFFECT OF NATURALLY INFECTED WITH HAEMOPARASITE TRYPANOSOMES AND AGE ON HAEMATOLOGICAL AND SERUM BIOCHEMICAL PARAMETERS OF INDIGENOUS WESTERN HIGHLAND GOAT, ETHIOPIA
In study area Gerura, South Western Shewa, Oromia Regional State of Ethiopia, goats are considered as one of the most important livestock. There is luck of information on effects of blood parasite Trypanosomes and age on...
PREVALENCE OF POTENTIAL NOSOCOMIAL BACTERIAL PATHOGENS IN LIQUID WASTE AND WASTE DUMP SOIL OF THREE MAJOR HOSPITALS IN CALABAR METROPOLIS, SOUTHSOUTH, NIGERIA
Hospital environment serves as a complex ecosystem that need proper intervention in order to have adequate infection control. The prevalence of potential nosocomial bacterial pathogens in liquid waste and waste dump soil...
SPECTROPHOTOMETRIC STUDIES OF SOME TRANSITION ELEMENTS AS COMPLEXES WITH NEW AZODYE 2- (SULFAPYRIDINE AZO)-4,5-DIPHENYL IMIDAZOLE
The yellow (λmax 440nm.) azodye ligand 2-(Sulfapyridine azo )-4,5- Diphenyl Imidazole (L) forms a stable red (λmax 480nm.), orange (λmax 480nm.) and red-violet (μmax 500nm.) color complexes with divalent cations cobalt,...
A REVIEW ARTICLE ON THE ORAL DOSAGE FORM: TABLETS
The most popular and easiest way to administered the drug by patients is tablets. They become popular because they were easy in preparation as compared to any other type of dosage form. The pharmacist prescribes the righ...