COMPARATIVE STUDY ON SOME BIOCHEMICAL PARAMETERS IN THREE SPECIES OF SALMONIDAE FAMILY FARMED IN BULGARIA
Journal Title: Водні біоресурси та аквакультура - Year 2018, Vol 1, Issue
Abstract
The purpose of the present project was to establish the physiological range of some blood biochemical parameters of the three most commonly used fish species of Salmonidae family in Bulgaria. The plasma biochemical profile was used as an indicator for fish welfare. At the same time the focus of this study was to identify any differences and similarities in biochemical indices in the blood of these three salmonids. Blood levels of biochemical parameters of three freshwater fishes, rainbow trout (Oncorhynchus mychiss), brown trout (Salmo trutta fario) and brook trout (Salvelinus fontinalis) were measured to establish the normal range, to serve as baseline data for evaluating health status. For the purposes of this project, 12 samples were obtained per each trout species from private fish farm within months October-November, when the water temperature was optimal for trout species (≈13.8 ̊C). Blood was drawn from the vena caudalis in heparinized containers. For biochemical analysis plasma were obtained by centrifugation (5 min at 3000 rpm) of samples. Plasma was kept frozen up to the time it was analyzed. Blood biochemical parameters were determined using a semi-auto analyser (Mindray BA-88, Shenzhen, China) with commercial diagnostic kits (Giesse, Diagnostics, Italy). The statistical analysis was performed using one-way analysis of variance (ANOVA). The results were processed with software Statistica v.10 (StatSoft Inc., 2002). In this study were analyzed concentrations of total protein, albumin, globulins, urea, creatinine, cholesterol, alanine aminotransferase, aspartate aminotransferase, calcium, phosphorus. The main aquaculture fish species in Bulgaria are the rainbow trout. Our findings do not show any significant differences for ALT activity and albumin values between rainbow and brook trout. Contrary, the mean calcium concentrations between these two species were the same. The obtained values of plasma globulins and phosphorus in rainbow trout were higher than other two fish species. The concentration of urea, creatinine, total protein, cholesterol and AST activity of rainbow trout were close of those reported for the brown trout. Results, obtained in this manuscript, revealed the baseline values and provide knowledge of some biochemical parameters of three of the most used species of Salmonidae family in Bulgaria. Recent results, although showing differences can be used as an important tool for monitoring of fish health and physiology condition. The data of the present investigation were similar to those found in previous studies of ours on the same three trout types wherein differences of some immunity parameters were determined as well.
Authors and Affiliations
D. Zapryanova, A. Atanasoff, G. Nikolov, T. Koynarski
Keywords
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