Comparing the Antimicrobial Potential of Sahara Honey from Algeria and Manuka Honey against Urogenital Microorganisms
Journal Title: Journal of Complementary and Alternative Medical Research - Year 2016, Vol 1, Issue 1
Abstract
Aims: Various studies have been conducted to investigate the antimicrobial properties of honey from different parts of the world. To date; no extensive studies of the antimicrobial properties of Sahara honey (SH) on urogenital microorganisms have been conducted. The objectives of this study were to conduct such studies and to compare the antimicrobial activity of SH with Manuka honey (MH). Place and Duration of Study: This study was conducted in the experimental laboratories at Pharmacognosy and Api-Phytotherapy Research Laboratory, Mostaganem University, Algeria, between April to May 2015. Methodology: Several unifloral SH and MH were analyzed to determine their total phenolic, color and antimicrobial capacities. The Folin-Ciocalteu assay was used to measure phenol content. Two different assays were performed to evaluate the antimicrobial potential of the honey samples: agar-well and disk diffusion assay. The honey samples were tested without dilution, and at 50 and 25% (w/v) dilution. Results: The means of the total phenolic contents of SH and MH were 82.8±0.23 and 143.5±0.62 mg/100 g honey as Gallic acid equivalent, respectively. Initial screening with the agar-well and disk diffusion assay demonstrated that undiluted honey had greater antimicrobial activity against all isolates tested. The zones of inhibition values of SH and MH against different strains ranged from 15 to 27.5 mm and 16.5–24 mm respectively. In addition, honey showed inhibition zone larger toward entire isolates when mixed. This is the first report on antimicrobial effect of SH against urogenital microorganisms. Conclusion: This work demonstrates the potential of Sahara honey is a very good trend in the treatment for polymicrobial infections.
Authors and Affiliations
Moussa Ahmed, Saad Aissat, Mohamed Amine Aissa, Noureddine Djebli
Keywords
Related Articles
- EP ID EP350623
- DOI 10.9734/JOCAMR/2016/27608
- Views 61
- Downloads 0
Long Term Consumption of Coconut Oil Diet Increased Anxiety Related Behaviour in CD1 Mice
The effect of prolonged consumption of virgin coconut oil (VCO) diet on anxiety was assessed in CD1 mice using the Open field (OF), Elevated plus maze (EPM) and Light/dark box (LD box) tests. Thirty male CD1 mice (28.4 -...
Phytochemical Screening and Antibacterial Activity of Prunus avium Extracts against Selected Human Pathogens
Aim: This research was carried out to determine the phytochemical properties and antimicrobial activities of leaf and stem bark ethanol extracts of Prunus avium L. against selected human pathogens. Methodology: The metho...
Chemical Constituents and Nutrient Composition of Carica papaya and Vernonia amygdalina Leaf Extracts
The present study evaluates the chemical constituents and nutrient composition of leaf extracts of Carica papaya and Vernonia amygdalina. The chemical constituents were analysed using gas chromatography mass spectrometry...
Phytochemical Analysis and Anticholinergic Properties of Methanol Leaf Extract of Arachis hypogea on Isolated Rabbit Jejunum
The acute toxicity and parasympatholytic properties of methanol extract of Arachis hypogea leaves were studied in vitro and in vivo. Qualitative phytochemical screening revealed the presence of alkaloids, glycosides, fat...
Perceptions and Practices on the Use of Moringa oleifera Lam: A Qualitative Study amongst Elderly Mauritians
The multifaceted properties of Moringa oleifera have spurred renewed interest into exploiting its nutritional values in the developing countries. In Mauritius, however, Moringa oleifera is fast losing its culinary value...