Comparing the Effect of Irrigation With 70% Isopropyl Alcohol, Distilled Water and Saline to Remove the Residual Sodium Hypochlorite before Irrigation with Chlorhexidine in Root Canal Therapy – An In Vitro Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 6
Abstract
Aim: The aim of this study is to compare the effect of different intermediate irrigant in removing residual sodium hypochlorite before irrigating with chlorhexidine by elemental analysis of precipitate formed by the residual sodium hypochlorite using Environmental Scanning Electron Microscopic Energy Dispersive X-Ray Spectroscopy (ESEM-EDS) system. Methodology: Forty extracted single rooted premolars were decoronated and divided into four groups GROUP I (positive control): The irrigation of the canal was done in the sequence 5 ml of 17% EDTA 5 ml of 5% NaOCl 5 ml of 2% chlorhexidine (CHX). GROUP II (70% isopropyl alcohol): The irrigation of the canal was done with 5 ml of 17% EDTA 5 ml of 5% NaOCl, 5 ml of 70% isopropyl alcohol as intermediate irrigant 5 ml of 2% CHX. GROUP III (distilled water): The irrigation of the canal was done with 5 ml of 17% EDTA 5 ml of 5% NaOCl, 5 ml of distilled water as intermediate irrigant 5 ml of 2% CHX. GROUP IV (normal saline). The irrigation of the canal was done with 5 ml of 17% EDTA 5 ml of 5% NaOCl, 5 ml of 0.9% normal saline as intermediate irrigant 5 ml of 2% CHX. Using a chisel and mallet, the roots were split longitudinally. One half of the split tooth was selected for the examination of smear layer under SEM. Five samples from the remaining ten halves of each group was taken for ESEM- EDX examination Results: The deposition of the precipitate layer was scored according to the criteria for evaluating smear layer given by Gutmann et al. Kruskal Wallis ANOVA was employed to compare precipitate scores between different groups and teeth levels. There is no statistically significant difference (P>0.05) between the three levels of the Group I. There is a highly significant difference (P<0.001) between the three levels of the teeth of Group II (Isopropyl alcohol group). There is a significant difference (P<0.05) between the levels of the teeth of Group III (Distilled water) and Group IV (Normal saline). There is highly statistically significant difference (P<0.001) in the scores between the irrigants in the coronal and middle third of the root canals. There is a significant difference in between the groups in the apical third (P<0.05). The elements present in the root canal wall of each group recorded and subjected to Analysis of variance (One Way ANOVA) was performed as parametric test to compare different groups as well as different teeth sites. For all statistical evaluations, a two-tailed probability of value, P< 0.05 was considered significant. Conclusion: Isopropyl alcohol removed more residual sodium hypochlorite from the root canal leaving minimum precipitate occluding the dentinal tubules. Elemental analysis of the precipitate showed presence of chlorine. In coronal and middle third contains more chlorine than apical thirds. Isopropyl alcohol removed more chlorine from the root canal.
Authors and Affiliations
Dr Padmaja Singh, Dr Luguram Tudu, Dr Vimal Singh Munda, Dr Rakesh Kumar
Keywords
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- EP ID EP600698
- DOI 10.9790/0853-1606031321.
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