COMPARISON AND PCR CONFIRMATION OF ENTERIC PROTOZOAL ISOLATES IN AIDS PATIENTS- OPTIMIZING DIAGNOSTIC APPROACH IN DIFFERENT RESOURCE SETTINGS
Journal Title: International Journal of Microbiology Research - Year 2016, Vol 8, Issue 2
Abstract
Opportunistic infections in AIDS patients are a harbinger of death. In this study presence of enteric protozoa (Cryptosporidium parvum, Cyclospora cayetanensis, Enterocytozoon bienuesi) as causative agents of diarrhea in HIV/AIDS patients were detected from their fecal samples. The conventional staining methods used for identification which had been employed earlier were compared with PCR results and different attributes (sensitivity, cost, hands on time, ease of use and batch testing) while performing investigations in the different set ups viz. rural, urban government, urban private and hospital cum research/referral centers were prioritized. Testing the samples by PCR gave 100% sensitivity and specificity. Since PCR is costly, a combination of at least two methods be employed in resource limited settings to avoid missing any parasite. This is the first study from India wherein a PCR was employed to confirm the presence of all the three above mentioned protozoa and application of Analytical Hierarchy Process was done.
Authors and Affiliations
LEKHA TULI, SUJEET RAI, D. K. SINGH, T. M. MOHAPATRA, A. K. GULATI
Keywords
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