Comparison between fish and linseed oils administered orally for the treatment of experimentally induced keratoconjunctivitis sicca in rabbits
Journal Title: Open Veterinary Journal - Year 2017, Vol 7, Issue 3
Abstract
The objective of this study was to compare the efficacy of two sources of omega 3 and 6, fish oil (FO) and linseed oil (LO), orally administered, alone or in combination, for treating experimentally induced keratoconjunctivitis sicca (KCS) in rabbits. Twenty-eight New Zealand rabbits were used in this study. Seven animals were allocated to the C group (negative control), and KCS was induced in 21 animals by topically applying 1% atropine sulfate drops for 7 days. Treatment with atropine was maintained throughout the study period (12 weeks). The rabbits were divided into 3 treatment groups containing 7 animals each: FO group, LO group and FLO group (FO and LO). The animals were evaluated using the Schirmer Tear Test I (STT I), Rose Bengal Test (RBT), fluorescein test (FT), tear film break-up time (TBUT), and conjunctival and histopathological analysis. There was a significant increase in STT I and TBUT values in treatment groups, but the increase occurred earlier in the FO group. The results of the RBT and FT were similar among treatment groups, except FT, in the FLO group, negative staining was only in 12 weeks. There was a significant decrease in the number of goblet cells in the FLO group compared with the other groups. The results demonstrated that orally administered of FO and LO improved the clinical signs of KCS. However, improvement occurred earlier in the FO group. Using oils in combination did not provide additional benefits. These results contribute to the future development of new oral formulations as adjuvant therapies for KCS.
Authors and Affiliations
Danielle Alves Silva| Animal Science Post Graduate Program, Oeste Paulista University (UNOESTE), Presidente Prudente, SP, Brazil, Gisele Alborgetti Nai| Department of Anatomy Pathology, Faculty of Medicine (UNOESTE), Brazil, Rogério Giuffrida| Faculty of Veterinary Medicine (UNOESTE), Brazil, Rafael Cabral Barbero| Faculty of Veterinary Medicine (UNOESTE), Brazil, Jacqueline Marcussi Pereira Kuhn| Faculty of Veterinary Medicine (UNOESTE), Brazil, Andressa Caroline da Silva| Faculty of Veterinary Medicine (UNOESTE), Brazil, Ricardo Henrique Zakir Pereira| Faculty of Veterinary Medicine (UNOESTE), Brazil, Maria Fernanda Abbade| Faculty of Veterinary Medicine (UNOESTE), Brazil, Luiz Felipe da Costa Zulim| Resident in Small Animal Medicine of the Veterinary Hospital (UNOESTE), Brazil, Carolina Silva Guimarães Pereira| Resident in Small Animal Medicine of the Veterinary Hospital (UNOESTE), Brazil, Silvia Franco Andrade| Department of Small Animal Medicine of the Veterinary Hospital (UNOESTE), Brazil
Keywords
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