Comparison between Light microscopy and LED microscopy in a tertiary care teaching institute in Kolkata – a pilot study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 10
Abstract
Background: Tuberculosis (TB) continues to be a grave public health problem in India and across the world. Sputum microscopy is the most reliable, easy and practical method to diagnose TB. With advent of new technology Light Emitting Diode (LED) microscopes have replaced light microscopy as the main tool for diagnosis. Aims: In this study, we evaluated efficiency of LED microscopy over light microscopy in diagnosis of TB through sputum smear. Methods and Materials: The study was conducted in the designated microscopy centre (DMC) of Calcutta Medical College under department of Pulmonology. LED microscopy was introduced in August, 2012. Data from TB registrar was retrospectively collected from January, 2009 to December, 2016. Data analysed for overall efficiency of LED microscopy over light microscopy in respect to average new TB suspects examined, total slide examined and slide positivity rate. Statistical Analysis Used: To compare the efficiency of both the methods, comparison of two means are statistically calculated by two sample t-test along with calculation of p value and Confidence Interval (CI). Results: LED microscopy has shown better efficacy in increasing average number of new TB cases examined as well as total slides examined per month. Light microscopy and LED microscopy are similar in achieving slide positivity but given the load of TB patients in India, LED microscopy proved to be time saving and a better alternative to light microscopy. Conclusion: Sputum smear microscopy is faster with LED microscope resulting into more number of new cases and more number of sputum slides to be examined within a given period of time.
Authors and Affiliations
Dr. Sudipta Pandit, Dr. Debabrata Saha, Dr. Subhajit Ghosh, Dr. Avradip Santra, Dr. Amitabha Sen
Keywords
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- EP ID EP239246
- DOI 10.9790/0853-1610030106
- Views 111
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