Comparison between the Mycob.T scanner system and manual microscopy in detecting acid-fast bacilli in sputum specimens
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 1
Abstract
ObjectiveTo compare the efficacy between the Mycob.T scanner system and manual microscopy for detecting acid-fast bacilli in sputum specimens.MethodsBetween January and November 2020ï¼ a total of 1 519 sputum samples from suspected primary tuberculosis patients from 5 designated tuberculosis hospitals in Shanghai were examined by Smear and BACTEC MGIT 960 liquid culture ï¼liquid cultureï¼ methods. Each specimen was subiected to 2 direct smear slides. One slide was stained by Z-N method and examined with manual microscopic method. Another slide was stained and scanned by the Mycob.T system. The efficacy of manual microscopy and the Mycob.T scanner system for detecting acid-fast bacilli in sputum specimens was compared based on the result of liquid culture. Results of the repetitive scanning by the Mycob.T scanner system and the recheck of the manual microscopy were analyzed.ResultsThe average positive rate by the Mycob.T scanner system was 14.4% ï¼219/1 519ï¼ while the average positive rate by manual microscopy was 16.3% ï¼248/1 519ï¼. No significant difference was observed ï¼Ï2=2.13ï¼ P=0.145ï¼. Based on liquid culture confirmation resultsï¼ the sensitivity of manual microscopy ï¼60.36%ï¼ was higher than that of the Mycob.T scanner system ï¼52.94%ï¼ï¼ and the difference is statistically significant ï¼Ï2=4.38ï¼ P=0.036ï¼. Both methods had high specificity ï¼98.94%ï¼. The concordance of the Mycob.T scanner system and manual microscopy was 95.46%ï¼ with the kappa value of 0.826. The results of repeatability test of the Mycob.T scanner system and the recheck results of the manual microscopy showed that the coincidence rate of scanning by the Mycob.T scanner system was 99.5% ï¼436/438ï¼ï¼ and the recheck coincidence rate by the manual microscopy was 98.6% ï¼432/438ï¼.ConclusionThe Mycob.T scanner system have high specificity for detecting acid-fast bacilli in sputum samples and good consistency with the results of manual microscopy. Compared with manual microscopic examinationï¼ the Mycob.T scanner system can greatly alleviate the work intensity.
Authors and Affiliations
LI Jing,DAI Wanqin,YU Chenlei,YUAN Feng,WANG Lili,SHEN Xin,JIANG Yuan,
Keywords
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