Comparison effect of ethanolic seed extract of Melia Azedarach L. (Meliaceae) against two Aphid species
Journal Title: Journal of Herbal Drugs - Year 2011, Vol 2, Issue 4
Abstract
Background & Aim: Aphids are of serious pests and vectors of multitude of viral diseases of field crops, vegetables, and greenhouse crops. They have displayed a remarkable ability to establish resistance to almost every insecticide with which it has been treated. To reduce the damage of these pests and dependence on the sometimes unwise use of synthetic pesticides in crop and vegetable plantations, the toxicity of ethanolic seed extract of Melia azedarach L. (Meliaceae) was assayed against 3-4 days-old individuals of Aphis gossypii Glover and Myzus persicae (Sulzer) (Hemiptera: Aphididae). Experimental: In this study, M. azedarach L. seeds were collected from Kerman, Iran. They were milled and powdered and extracted with ethanol (95%) solvent. All experiments with different concentrations were conducted by topical test bioassay in laboratory. The treated aphids were reared at 25±1 °C temperature, relative humidity of 60±10 % and 16 hours of artificial light at an intensity of about 4000 lux. Ethanol (95%) was used as control treatments. Results & Discussion: The results indicated that in the concentration 60 µl/ml, the mortality of A. gossypii treatment after 24 h was 95% and it was significantly higher than M. persicae treatment (p<0.005). Also the LC50 of ethanol seed extracts of M. azedarach for A. gossypii and M. persicae after 24 hr was 13.7 and 15.0 µl/ml, respectively. It is concluded that the ethanolic seed extract of M. azedarach may be applicable as a safe insecticide to aphid's control in IPM programs.
Authors and Affiliations
Elham Salari; Kamal Ahmadi; Reza Zamani Dehyaghobi
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