Comparison of detection methods for extended-spectrum beta-lactamases in Escherichia coli strains
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2014, Vol 68, Issue
Abstract
Introduction: Detection of extended-spectrum beta-lactamases (ESBLs) could be a major challenge for microbiologists – the difficulties arise mainly from the phenotypic differences among strains.Materials and Methods: Evaluation of ESBLs was performed on 42 strains of E. coli by: 1) DDST on MHA, 2) DDST on MHA with cloxacillin, 3) CT on MHA, according to CLSI, 4) CT on MHA with cloxacillin, 5) Etest ESBL (AB Biodisk), 6) CHROMagarTM ESBL (GRASO), 7) ChromID® ESBL (bioMérieux), and 8) automatic system VITEK2 ESBL test (bioMérieux).Result: Positive results were obtained for 20 strains using method 1, for 18 strains using method 2, 17 by method 3, 14 by method 4, 11 by method 5, 39 by method 6, 40 by method 7, and 15 by method 8. Using Etest ESBL 6.0 non-determinable results were obtained. The most consistent results were obtained when comparing the results of method 3 with results of method 2 (97.6%), and comparing the results obtained using methods 3 and 8 (95.2%).Conclusions: Based on our study we conclude that the chromogenic media can only be used as a screening method for the detection of ESBLs in E. coli rods. Etest is less useful compared to other phenotype methods, due to the impossibility of obtaining results for all the tested strains. Adding cloxacillin to MHA does not increase the frequency of detection of ESBLs in E. coli strains. DDST seems to be the most reliable among phenotypic methods for the detection of ESBLs in E. coli rods.
Authors and Affiliations
Ewelina Kałużna, Patrycja Zalas-Więcek, Eugenia Gospodarek
Keywords
Related Articles
- EP ID EP67415
- DOI -
- Views 155
- Downloads 0
18 kDa translocator protein – implications in cell’s functions
The mitochondrial 18kDa Translocation Protein (TSPO) was first identified in 1977 by its capability to bind benzodiazepines in peripheral tissues. It is more commonly known after its previous name – peripheral benzodiaze...
Effect of vitamin D receptor gene (VDR) polymorphism on body height in children – own experience
Genetic and environmental factors have an influence on the process of growth and development of the body. One of numerous genetic factors can be the vitamin D receptor gene (VDR). The study aimed at evaluating the relati...
Tiamina i jej pochodne w regulacji metabolizmu komórek
Tiamina (wit. B[sub]1[/sub]) od ponad 70 lat cieszy się niesłabnącym zainteresowaniem biologów, biochemików i lekarzy ze względu na swój udział w głównych procesach biochemicznych i fizjologicznych. Cząsteczka tiaminy...
Synthetic lethality as a functional tool in basic research and in anticancer therapy
Nowadays, cancer and anticancer therapy are increasingly mentioned topics. Groups of researchers keep looking for a tool that will specifically and efficiently eliminate abnormal cells without any harm for the normal one...
Blood groups – minuses and pluses. Do the blood group antigens protect us from infectious diseases?
Human blood can be divided into groups, which is a method of blood classification based on the presence or absence of inherited erythrocyte surface antigens that can elicit immune response. According to the International...