Comparison of detection methods for extended-spectrum beta-lactamases in Escherichia coli strains
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2014, Vol 68, Issue
Abstract
Introduction: Detection of extended-spectrum beta-lactamases (ESBLs) could be a major challenge for microbiologists – the difficulties arise mainly from the phenotypic differences among strains.Materials and Methods: Evaluation of ESBLs was performed on 42 strains of E. coli by: 1) DDST on MHA, 2) DDST on MHA with cloxacillin, 3) CT on MHA, according to CLSI, 4) CT on MHA with cloxacillin, 5) Etest ESBL (AB Biodisk), 6) CHROMagarTM ESBL (GRASO), 7) ChromID® ESBL (bioMérieux), and 8) automatic system VITEK2 ESBL test (bioMérieux).Result: Positive results were obtained for 20 strains using method 1, for 18 strains using method 2, 17 by method 3, 14 by method 4, 11 by method 5, 39 by method 6, 40 by method 7, and 15 by method 8. Using Etest ESBL 6.0 non-determinable results were obtained. The most consistent results were obtained when comparing the results of method 3 with results of method 2 (97.6%), and comparing the results obtained using methods 3 and 8 (95.2%).Conclusions: Based on our study we conclude that the chromogenic media can only be used as a screening method for the detection of ESBLs in E. coli rods. Etest is less useful compared to other phenotype methods, due to the impossibility of obtaining results for all the tested strains. Adding cloxacillin to MHA does not increase the frequency of detection of ESBLs in E. coli strains. DDST seems to be the most reliable among phenotypic methods for the detection of ESBLs in E. coli rods.
Authors and Affiliations
Ewelina Kałużna, Patrycja Zalas-Więcek, Eugenia Gospodarek
Keywords
Related Articles
- EP ID EP67415
- DOI -
- Views 136
- Downloads 0
Selenium in Graves' disease
The aim of this study was to present the current state of knowledge of the role of selenium in Graves’ disease. Recently, in the pathogenesis and course of this autoimmune disease, more attention has been paid to the rel...
Eradication of Rinderpest from Poland in 1921-1922
Due to Rinderpest disease (cattle plague) in Poland, over 40,000 cattle died within the three months of the first half of 1920. Stopping the epizooty of rinderpest (pestis bovum; cattle plague; Typhus bovum contagiosus)...
The presence of some cytokines and Chlamydia pneumoniae in the atherosclerotic carotid plaque in patients with carotid artery stenosis
Background: Over the last few years the role of microorganisms in the pathogenesis of atherosclerosis has been widely discussed. Chlamydia pneumoniae activates immune cells to produce cytokines that are responsible for t...
Arsenic trioxide: impact on the growth and differentiation of cancer cells and possible use in cancer therapy
Arsenic trioxide (As2O3) has recently been identified as an effective drug in different types of cancer therapy. It is a useful pharmacological agent in acute promyelocytic leukemia (APL) treatment, especially the form t...
Rzadkie kliniczne odmiany glejaka wielopostaciowego
Glejak wielopostaciowy, zaliczany do najwyższego stopnia złośliwości histologicznej według Światowej Organizacji Zdrowia, jest najczęstszym nowotworem pierwotnym OUN. Rozwija się głównie u osób dorosłych, umiejscawia zwy...