Comparison of different deafening strategies based on ototoxic drugs on mouse animals model
Journal Title: Otolaryngologia Polska - Year 2012, Vol 66, Issue 1
Abstract
SUMMARY Aim of the study: To compare safety, reliability and usefulness of two deafening protocols on animal mouse model, based on aminoglycosides exposure Material and method: Adults mice, Bulb/C, deafened with kanamycine 14 days treatment (group I), single kanamycin injection followed by etacrinic acid administration (group II) and control group. Hearing evaluation performed with ABR recordings on 6th day after drug exposure Results: Both protocols were not able to guarantee complete ablation of the inner ear in tested animals. Although short deafening strategy was more effective (83.33% deaf mice) it was combined with high rate of mortality during general anesthesia for hearing evaluation. Conclusions: Variable outcomes in deafening mouse animal model implies the necessity of hearing evaluation every time prior to the pathophysiological as well as molecular studies. Mice exposed to severe oto- and nephrotoxic insult do not recover after anesthetic drug administration, thus harvesting inner ear tissues especially as the source of RNA should be performed immediately after ABR recordings.
Authors and Affiliations
Maciej Wróbel, Michał Karlik, Marcin Szaumkessel, Małgorzata Rydzanicz, Krzysztof Szyfter, Witold Szyfter
Keywords
Related Articles
- EP ID EP52437
- DOI -
- Views 112
- Downloads 0
Morfologia głośni i charakterystyka percepcyjno-akustyczna głosu i mowy po laryngektomii fronto-lateralnej poszerzonej z następową epiglotoplastyką
For optimalisation glottis morphology and its phonatory function after frontolateral laryngectomy (FLL) the reconstruction of larynx is made; in Clinic of Otolaryngology the most common is using epiglottis wit or without...
Universal Neonatal Hearing Screening Program in Poland – 10-year summary
Introduction: The analysis was aimed to summarize the outcomes of Polish Universal Neonatal Hearing Screening Program (PUNHSP). Materials and methods: The analysis comprised the population of children registered in the P...
Efekty chirurgicznego leczenia u pacjentów z rowkiem głośni
Rowek głośni jest jedną z najtrudniejszych do leczenia łagodnych zmian patologicznych fałdów głosowych. Ford wyróżnia trzy typy rowka: I- powierzchowny, II –vergeture oraz III -true sulcus vocalis. Cel: Ocena efektów le...
Assessment of auditory processing in children with dyslalia
The objective of the work was to assess occurrence of central auditory processing disorders in children with dyslalia. Material and method: The material included 30 children at the age 7–9 years old being under long-term...
Rhinophyma – leczenie za pomocą lasera CO2
Treatment of the advanced form of rhinophyma causing severe nasal deformity and nasal breathing impairment is reserved mainly for surgical methods. Laser techniques among them play particular role. The aim of the study w...