Comparison of different deafening strategies based on ototoxic drugs on mouse animals model
Journal Title: Otolaryngologia Polska - Year 2012, Vol 66, Issue 1
Abstract
SUMMARY Aim of the study: To compare safety, reliability and usefulness of two deafening protocols on animal mouse model, based on aminoglycosides exposure Material and method: Adults mice, Bulb/C, deafened with kanamycine 14 days treatment (group I), single kanamycin injection followed by etacrinic acid administration (group II) and control group. Hearing evaluation performed with ABR recordings on 6th day after drug exposure Results: Both protocols were not able to guarantee complete ablation of the inner ear in tested animals. Although short deafening strategy was more effective (83.33% deaf mice) it was combined with high rate of mortality during general anesthesia for hearing evaluation. Conclusions: Variable outcomes in deafening mouse animal model implies the necessity of hearing evaluation every time prior to the pathophysiological as well as molecular studies. Mice exposed to severe oto- and nephrotoxic insult do not recover after anesthetic drug administration, thus harvesting inner ear tissues especially as the source of RNA should be performed immediately after ABR recordings.
Authors and Affiliations
Maciej Wróbel, Michał Karlik, Marcin Szaumkessel, Małgorzata Rydzanicz, Krzysztof Szyfter, Witold Szyfter
Keywords
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