COMPARISON OF DIFFERENT PHENOTYPIC METHODS FOR AmpC DETECTION
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 26
Abstract
BACKGROUND AmpC -lactamases are clinically important cephalosporinases which confer resistance to cephamycins, penicillins, aminoglycosides and β-lactam-β-lactamase inhibitor combinations and produced in several Enterobacteriaceae. The aim of this study was to compare various phenotypic detection methods of AmpC β-Lactamases. MATERIALS AND METHODS This was a descriptive study. Three hundred multidrug-resistant bacterial isolates were tested for AmpC β-lactamase enzyme production. Cefoxitin (30 μg) resistance was used as the screening test for AmpC enzyme detection. Phenotypic confirmation was done by conventional disk combination method (Cefoxitin/ cefoxitin + boronic acid), 3D extract method, double disk synergy test (DDST) and AmpC disk test. RESULTS All 300 strains were found to be AmpC positive by the screening test. Out of these 300 strains, 100 strains were positive by disk combination method, 107 strains by DDST, 111 strains by 3D extract method and 170 strains by AmpC disk test. CONCLUSION Cefoxitin resistance can be used as the screening test for AmpC. AmpC disc test is simple to perform. Many strains can be checked on single petri plate and get best results in comparison to other methods of AmpC detection. So, it can be used as a phenotypic method for detection of AmpC enzyme production in resource-limited settings, where genotypic detection methods are not available.
Authors and Affiliations
Mohd. Rashid, Huma Naim, Indu Shukla
Keywords
Related Articles
- EP ID EP466483
- DOI 10.14260/jemds/2018/681
- Views 86
- Downloads 0
ANATOMY OF RECURRENT LARYNGEAL NERVE IN THYROIDECTOMY
BACKGROUND Thyroid surgeries have been through tremendous evolution to make it a safe procedure. Thyroid surgery can today be performed with practically no added mortality other than the risk of general anaesthesia. A li...
STRANGULATED LITTRE’S HERNIA: CASE REPORT AND REVIEW OF LITERATURE
Littre's hernia is uncommon clinical entity. We are presenting a case of Strangulated Littre's Hernia. A 19 years old male, came with pain and swelling in right inguinal region, which turned out to be an obstru...
A RARE PRESENTATION OF CEREBROVASCULAR INSUFFICIENCY
Locked in syndrome refers to a neurological condition associated with infarction of the ventral pons. The syndrome is manifested by quadriplegia, lower cranial nerve paralysis and mutism with preservation of onl...
ANGANWADI SERVICE UTILISATION IN A DISTRICT OF NORTH KERALA, INDIA
BACKGROUND The Integrated Child Development Services Scheme represents one of world’s largest and unique programme for early childhood development. Preschool children call for focused attention in India because India has...
RESULTS OF CLOSED INTRAMEDULLARY NAILING VS PLATE OSTEOSYNTHESIS IN DIAPHYSEAL FRACTURES OF BOTH BONES FOREARM IN ADULTS
[b]BACKGROUND[/b]: Plate osteosynthesis is the most commonly used technique for the treatment of diaphyseal forearm fractures in adults. However, application of a plate can disrupt the periosteal blood supply...