Comparison of Different Phenotypic Tests for Detection of ESBL (Extended Spectrum Beta Lactamases) Producing Pseudomonas Aeruginosa
Journal Title: Indian Journal of Cancer Education and Research - Year 2017, Vol 5, Issue 1
Abstract
Background: P. aeruginosa is the most common nosocomial pathogen encountered and a known organism causing high mortality and morbidity. Due to indiscriminate antibiotic use, resistance is very commonly known in them and ESBL enzyme production being the most predominant one. Material and Methods: A total of Ninty isolates of P. aeruginosa were tested for the presence of ESBL enzyme by both disc diffusion and double disc synergy test. Antibiotic sensitivity pattern of ESBL-positive P. aeruginosa was determined. Results: Of the 1200 pus samples screened, 90 isolates of pseudomonas were tested for ESBL production. Of the 90 P.aeruginosa isolates, 57 (63%) were sensitive to 3GC and 33 (37%) were resistant.Of the 33 P.aeruginosa resistant to ceftazidime, DDT detected 9 (27%) of ESBL producers and DDST detected 17 (51%). And 17 (51%) did not show ESBL production by either of the methods used in the study. All the ESBL-positive P. aeruginosa were multi-drug resistant, with 100% sensitivity to imipenem; followed by ofloxacin (70%). Conclusion: From this study, we conclude that DDST proved to be better method than DDT to detect ESBL producing P. aeruginosa. However in the absence of any CLSI guidelines for detection of ESBL in Non-fermenters, we reframe from commenting on specificities of either of tests. There is a strong need for standardization/ CLSI guidelines for detection.
Authors and Affiliations
Soumya S
Keywords
Related Articles
- EP ID EP462581
- DOI 10.21088/jmrr.2395.6623.3217.8
- Views 117
- Downloads 0
Drug Utilization Review of Anticancer Drugs in Medical Oncology Department of a Tertiary Care Teaching Hospita
The present study aimed to analyze the drug utilization pattern of anticancer drugs in medical oncology department of a tertiary care teaching hospital, Tamilnadu. Each prescription in the department of medical oncology...
Comparision of Carcinoma Cervix Treated with Conventional vs Intensity Modulated Radiotherapy with Concurrent Chemotherapy
Introduction: Cervical cancer is the fourth most common cancer in women, and the seventh overall, Use of IMRT in pelvic malignancies has shown reduced radiation exposure to adjacent bowel and bladder. IMRT is superior to...
Speciation and Resistotyping of Coagulase Negative Staphylococci in Clinical Isolates in Kerala Medical College, Palakkad
Introduction: Coagulase negative staphylococci are important causative agents of 10% of pyogenic infections in hospitals, which include a range of infections like surgical wound sepsis, bacteremia, native valve endocardi...
Cytological Grading of Breast Carcinoma on FNAC & Correlation with Histological Grading on Excised Specimen
Background: With the advent of fine needle aspiration cytology (FNAC), the approach to diagnosis and management of breast lesions has been revolutionized. Assigning a tumor grade to breast cancer provides important progn...
Hypoxia Inducible Factor-1 (HIF-1) and Cancer Progression: A Comprehensive Review
Hypoxia is characterized as a decrease oxygen levels in tissue, represents a fundamental pathophysiological condition in the microenvironment of solid tumors. The key component of hypoxia sensing in the cell is the hypox...