Comparison of immunofluorescence assay (IF) with ELISA in detection of antinuclear antibodies
Journal Title: Indian Journal of Pathology and Oncology - Year 2018, Vol 5, Issue 3
Abstract
Patients with autoimmune connective tissue diseases often have antibodies that are directed against multiple nuclear antigens called as antinuclear antibodies ANA Two testing methods ie ELISA and indirect immunofluorescence IF techniques are used to detect these antibodies Though ELISA is a cheaper method IF is a preferred method for detection of ANA In our study we have compared these two techniques for their diagnostic performance Both the testing methods were applied on 155 samples Of these 135 samples were from test group and 20 samples were controls negative and positive controls Of the 135 test samples IF yield positive results in 251851 cases and was found negative in 1108149 cases Positive results were found by ELISA in 201481 cases and negative in 1158519 cases Samples showing positive results with both methods were 181333 and samples showing negative results with both methods were 108800 07518 cases that gave negative results by ELISA were found to be positive by IF 02148 samples that were found to be positive by ELISA were negative by IF Sensitivity specificity of ELISA was compared with IF and was found to be 900 and 939 respectively From this study it can be concluded that for testing ANA IF is better than ELISAKeywords IF Immunofluorescence ELISA Enzyme Linked Immunosorbent Assay ANA Antinuclear Antibodies
Authors and Affiliations
Ashish Tayde, Chetna Agrawal, A. T. Deshmukh
Keywords
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- EP ID EP475914
- DOI 10.18231/2394-6792.2018.0081
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- Downloads 0
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