COMPARISON OF LABORATORY METHODS FOR THE DIAGNOSIS OF URINARY TRACT INFECTION
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2013, Vol 1, Issue 6
Abstract
Introduction: Urinary Tract Infection (UTI) is most frequent bacterial infection.Conventional urine culture is widely used for its diagnosis. This method is time consuming, expensive and patients are often treated before results are available. Dipsticks Nitrite Test (NT), Leucocyte Esterase (LET) and microscopic examination are commonly used primarily to predict the outcome of urinary tract infection. Objective: To compare reagent strip testing with microscopy and culture on different medias to identify significant bacteriuria. Methods: 500 samples were tested by semi-quantitative culture on cysteine lactose electrolyte deficient agar (CLED) with Andrade indicator, Mac conkey agar , Nutrient agar, microscopic examination of urine for significant pyuria, dipstick LET and NT. Culture was used as gold standard to evaluate the performance of direct microscopy and dipstick tests Results: Out of 500 urine sample 162(32.4%) were culture positive , out of these 162 samples 100(61.7%) samples showed significant bacteriuria (>105 CFU/ml). Sensitivity, specificity, positive predictive value (PPV)and negative predictive value (NPV)of the dip-stick LET were 71.6%, 75.7%, 58.5%, and 84.7% respectively; those of the dip-stick NT were 59.2%, 84.0%, 64%, and 81%respectively; and those for microscopic significant pyuria detection were 66%, 81.3%,62.9% and 83.3% respectively. Highest sensitivity (87.6%) and NPV (93.1%) was obtained on combining microscopy and dip-stick LET and NT . Escherichia coli was the most common organism isolated (n75=46.3%). Conclusion: Study suggests that positive cultures cannot be accurately predicted by single method, but Combined use of all three methods have > 90% prediction (NPV) for negative culture. Use of CLED agar with Andrade indicator proven to be useful as primary medium & helped to reduce the plate burden & work load.
Authors and Affiliations
Dr. Sanjay D Rathod| Associate Professor,Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India, Dr. Dipti D Patel| 2ndYear resident ,Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India, Dr. Bimal Chauhan| Assistant Professor ,Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India, Dr. Jayshree D Pethani| Associate Professor,Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India, Dr. Setal chauhan| Tutor pathology (GMERS Gandhinagar) ,Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India, Dr. Parul D Shah| Professor & Head , Department of Microbiology, Smt. NHL Municipal Medical College, Ahmedabad, India
Keywords
Related Articles
- EP ID EP1715
- DOI -
- Views 481
- Downloads 50
LUBIPROSTONE: A NOVEL DRUG TO TREAT CONSTIPATION
Lubiprostone, a bicyclic fatty acid, is a novel drug of new class of agents called prostones. It is approved by US FDA for the treatment of chronic constipation and constipation associated irritable bowel syndrome (IBS-...
GENE THERAPY AND CARCINOMA PROSTATE
Despite recent advances in early detection and treatment, prostate cancer is the most common cancer and the second leading cause of cancer-related deaths in developed countries. Prostate cancer patients having a limited...
Vitilego Cure by Homoeopathy
Vitiligo known as leucoderma is a relatively common skin disorder, in which white spots or patches appear on the skin. These spots are caused by destruction or weakening of the pigment cells in those areas, resulting in...
Knowledge, attitude and practice of medicated contraception among females of reproductive age group
Lack of knowledge of contraception has lead to increased maternal mortality, morbidity due to unsafe abortion. Hence our study was aimed to determine knowledge, attitude and practice of contraception and impact of socio...
Enhancement of Hepatoprotective Effect of Herbal Formulation (F1) on Carbon tetrachloride and Ethanol Induced Liver Toxicity in Albino Rats
There are several plants and formulations which are recommended for the treatment of liver diseases. The formulation F1 was composed with Andrographis paniculata (Kalmegh), Boerhavia diffusa (Punarnava), Eclipta alba (Bh...