Comparison of Longitudinal and transverse sections of teeth in Assessing Cemental Annulations for Age Estimation using Bright Field and Phase Contrast Microscopy: An in-vitro pilot study.
Journal Title: International Journal of Dental Science and Innovative Research (IJDSIR) - Year 2019, Vol 2, Issue 3
Abstract
Background: Age estimation plays an important role in identification of an individual in the field of forensic odontology. Literature studies have revealed that tooth cemental annulations would serve as a reliable tool in establishing the age of an individual. The use of specialized microscopic methods in conjunction with longitudinal sections and transverse sections individually or in combination respectively has also being employed to enhance the assessment of the cemental annulations. Aims: The study aims at assessment and evaluation of cemental annulations in longitudinal and transverse sections of the extracted teeth using bright field and phase contrast microscopic methods. Materials and methods :Thirty teeth were sectioned longitudinally and thirty teeth were transverse-sectioned at mid portion of the root using diamond discs. Sections were mounted and observed under bright field microscope and phase contrast microscope. The cemental lines were assessed for age estimation by adding eruption age of that tooth to obtain the chronologic age for each individual. Results: The estimation of age using longitudinal sections is significantly better as compared to transverse sections. There was no significant difference in estimating age using bright field microscopy and phase contrast microscopy. Conclusion: The study concluded that, the longitudinal sections of the extracted teeth appeared to be more promising in comparison to the transverse sections using cemental annulations as parameter for estimating the age of an individual. The study did not reveal any significance difference between the two methods of microscopy. More so over, it would be advisable that larger sample size would shed more light on the obtained results.
Authors and Affiliations
Dr. Heena Zainab
Keywords
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