Comparison of Matrix Metalloproteinases 2 mRNA Expression in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia
Journal Title: International Journal of Medical Laboratory - Year 2016, Vol 3, Issue 4
Abstract
Background and Aims: Prostate cancer is the second most common cancer in men worldwide in men. Matrix metalloproteinase 2 (MMP2) has a role in the invasion and destruction of the basement membrane and the extra-cellular matrix and facilitating the process of tumor cell invasion. The present study was conducted to compare the expression of MMP2 gene in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Materials and Methods: 48 samples of PCa and 48 samples of BPH (as controls) were examined. RNA was extracted from paraffin tissue blocks and then used for cDNA synthesize. The real time polymerase chain reaction method was used to quantify the expression of MMP2 gene. The level of gene expression was assessed using participants’ clinicopathological characteristics. Results: The expression of MMP2 gene increased in the PCa samples compared with the BPH samples (p=0.003). MMP2 gene increased in all the grades of this group compared with the controls. The highest expression, however, was observed in the moderately differentiated grade. No significant correlations or relationships were observed in the PCa samples between prostate specific antigen and the other study variables such as age, MMP2 expression, disease grade and the Gleason score; however a positive correlation was observed between prostate specific antigen and the samples' pathological stage. Conclusions: The expression of MMP2 increased in the PCa samples compared with the BPH samples, with the highest expression occurring in the samples with a Gleason score of 6. Further tests and studies conducted with larger sample sizes may help to use this marker in differentiating malignant from benign samples.
Authors and Affiliations
Bahareh Rahmatizadeh, Farzaneh Tafvizi, Masoud Salehipoure
Keywords
Related Articles
- EP ID EP212515
- DOI -
- Views 114
- Downloads 0
Paradoxical Regulation of Copper and Zinc and Changes in Neurogenesis, Alcohol Preference and Salt Appetite in Isolated Male Rats
Background and Aims: Alcohol abuse is an important concern of many societies. Hippocampal neurogenesis regulates abusing drugs in a positive manner. The aim of this study was to identify factors that regulate neurogenesi...
RT-PCR Detection of Coxsackievirus B3: A Viral Myocarditis
Backgrounds and Aims: Coxsakievirus B3 (CVB3), one of the six Coxsakievirus B serotypes, is a member of the Enterovirus genus within the Picornaviridae family. CVB3 is an important pathogen of viral myocarditis, which ac...
Comparison the Occurrence Rate of Oral Candida Species in Edentulous Denture Wearer and Dentate Subjects
Background and Aims: Different Candida species, especially Candida albicans have been known as part of human oral cavity normal flora. Changes in the oral environment resulting from tooth loss or denture application can...
Anti-viral Effect and Mechanism of Carvacrol on Herpes Simplex Virus Type 1
Background and Aims: Herpes simplex virus type 1 (HSV-1) belonging to herpes viridae family is a normal human pathogen with benign lesions in immunocompromised patients which creates serious problems. Acyclovir is used t...
Effect of Ferula Assafoetida on Cytoplasmic Membrane Glucose Transporter Isotype-4 of C2C12 Cell Line
Background and Aims: Ferula Assafoetida is an antioxidant plant which has long been used in Iranian traditional medicine. Recently, it has been reported to have hypoglycemic and hyperinsulinemic effects, but the molecula...