Comparison of Methods of Biofilm Detection in Urinary Candida Isolates and Evaluating its Role in Persistent Candiduria
Journal Title: National Journal of Laboratory Medicine - Year 2018, Vol 7, Issue 2
Abstract
Introduction: Candiduria is a common finding in catheterised patients with increasing trend of non albicans Candida species. Biofilm formation on indwelling catheters leads to persistent colonisation ending up in infection in immunocompromised patients. Aim: This study was conducted with the aim of identification of Candida species isolated from urine samples of catheterised patients; comparison of three phenotypic methods for biofilm detection in these Candida isolates and evaluating the role of biofilm in persistent candiduria. Materials and Methods: A prospective study was conducted in Microbiology Department. Total 50 non repeat urine samples were selected from the patient’s sample received in the laboratory routinely. Samples with significant number of pus cells and budding yeast cells in direct microscopy and significant count of Candida species in culture from catheterised patients were subjected to HiChrom agar for species identification and tube method, congo red agar and tissue culture plate method for biofilm detection. Diagnostic test analysis was done for the three biofilm detection methods taking tissue culture plate methods as gold standard. Chi-square analysis was performed for comparison of persistent candiduria with duration of catheterisation and biofilm. Results: Sensitivity and specificity for tube method was 100% and 0% respectively and 26.53% and 100% respectively for Congo red agar. A significant difference was found in persistent candiduria in patients with duration of catheterisation of one week and those with more than one week (χ2 =0.0047 p-value=0.9) and also in proportion of biofilm positive Candida isolates in these two group of patients χ2 =4.56, p-value=0.9). Conclusion: Tube method showed excellent sensitivity while congo red agar had better specificity. This study shows that Candida colonisation and biofilm formation is associated with persistent candiduria.
Authors and Affiliations
Rup Jyoti Chandak, Bibhabati Mishra, Archana Thakur, Poonam Sood Loomba, Vinita Dogra
Keywords
Related Articles
- EP ID EP528924
- DOI 10.7860/NJLM/2018/34218:2287
- Views 124
- Downloads 0
Carcinosarcoma of Breast with Hormone Receptor and Her2/neu Positivity
Carcinosarcoma is an extremely rare tumor of the breast comprising of two distinct cell lines i.e. the epithelial and mesenchymal. It is a highly aggressive tumor with a poor prognosis compared to other breast cancers wi...
A Study of Vesiculobullous Lesions of Skin
ABSTRACT Introduction: Bullous dermatoses are a wide variety of blistering diseases some of which can be extremely debilitating and even fatal. This study shows the importance of histopathology over immunofluorescence st...
Correlation between Ferritin and Iron Overload in Heart and Liver in BetaThalassaemia Major Patients in Shahrekord, Iran
Introduction: Beta-thalassaemia is one of the severe types of thalassaemia with very high healthcare costs. Iron overload in heart and liver is the most fatal complication in betathalassemia. Aim: This study was performe...
Rosai Dorfman Disease: A Rare Cause of Cervical Lymphadenopathy
Rosai Dorfman disease, a sinus histiocytosis, is rare to find and difficult to diagnose. It may masquerade as commoner diseases including infections and malignancies. Imaging may yield ambiguous results and it is often t...
Clinicopathologic Study of Appendix Specimens- A Two Year Retrospective Study at a Tertiary Care Center
ABSTRACT Introduction: Acute appendicitis is the most common abdominal emergency and has remained an on going diagnostic challenge. Histopathologic studies are the gold standard for final diagnosis. Aim: To study the his...