Evaluation of Phenotypic Methods for Detection of Biofilm Formation in Uropathogens
Journal Title: National Journal of Laboratory Medicine - Year 2018, Vol 7, Issue 4
Abstract
Introduction: Urinary tract infection (UTI) is one of the leading causes of morbidity encountered in clinical practice. Biofilm produced by the urinary pathogens leads to recurrent and recalcitrant UTI there by contributing to longer stay in hospital and increased cost of treatment. Aim: The present study was conducted to evaluate Congo Red Agar method (CRA) and Tube Method (TM) in detection of biofilm formation in uropathogens with respect to Tissue Culture Plate method (TCP). Materials and Methods: Study was conducted in the Department of Microbiology from May 2016 to May 2017. Midstream clean catch urine collected from patients with symptoms of UTI was processed by standard methods. A total of 264 randomly selected urinary isolates were subjected to biofilm detection by CRA method, tube method and TCP method. TCP was considered as gold standard. Results were expressed in terms of percentages, sensitivity, specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV). Results: Biofilm was detected in 105 (39.77%) isolates by TCP method, in 124(46.97%) by CRA method and 101(38.26%) by tube method. CRA method and TM method showed a sensitivity of 80% & 63.81%, specificity of 75.47% & 78.62%, PPV of 68.29% & 66.34%, and NPV of 85.11% and 76.69% respectively. Conclusion: Congo red agar and tube methods can be considered for detection of biofilms in resource constraint conditions.
Authors and Affiliations
T A Dhanalakshmi, D Venkatesha, Aliya Nusrath, N Asharani
Keywords
Related Articles
- EP ID EP529288
- DOI 10.7860/NJLM/2018/35952:2321
- Views 120
- Downloads 0
A Study of Epidermal Growth Factor Receptor 2 (HER2/neu) Expression in Endoscopic Biopsies and Gastrectomy Specimens of Gastric Adenocarcinomas
Introduction: Gastric carcinoma (GC) is the fourth most commonly diagnosed cancer and has poor prognosis, since most of the patients present with advanced disease and the therapeutic options available are very few. The r...
Evaluation of Thrombocytopenia in Megaloblastic Anemia by Platelet Indices and Megakaryocytes- Comparison with Hypoproduction and Hyperdestruction
Introduction: Thrombocytopenia may result from many mechanisms such as: marrow hypoplasia (decreased megakaryocytes), ineffective thrombopoiesis (normal to increased megakaryocytes) and increased destruction of platelets...
Uncommon Papillary Lesions in Human Breast-Rare Pathology
Breast cancer is a lesion that usually affects female but the male community is not spared from being affected. The cause of male breast cancer is not clear but predisposing factors found are increased age over 50’s, fam...
Idiopathic Hypertriglyceridemia in Thalassemia Major: A Case Report
Thalassemia major is a severe hereditary haemolytic anaemia and is usually associated with normal serum lipid profile. But there are few reports in literature that hypertriglyceridemia can have an idiopathic association...
Cell Block- A Useful Adjunct in Cytopathology of Serous Effusions
Introduction: Use of the Cell Block (CB) technique in cytology increases the diagnostic accuracy. This technique enables cells to be retrieved in a fluid specimen to form a paraffin block, which concentrates the cells in...