Comparison of Phenotypic and molecular profile of Coagulase negative Staphylococci from clinical isolates and commensals along with Biofilm detection
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 2
Abstract
Background: Coagulase-Negative Staphylococci (CoNS) are commensals of the human skin and mucosa. However, Staphylococcus epidermidis (S. epidermidis) and other CoNS have emerged as nosocomial pathogens. This study aims to identify and characterize clinical and commensal isolates of CoNS, to compare their association with potential virulence factors and to determine their antimicrobial resistance pattern. Materials and Method: This study was conducted in Department of Microbiology of a tertiary care centre in Bangalore. A total of 100 samples were processed (50 samples isolated from clinical samples were treated as cases and 50 from skin of healthy volunteers were considered as controls).Identification and speciation of CoNS was done by conventional biochemical tests. Antimicrobial susceptibility was tested by Kirby Bauer disc diffusion method. Detection of biofilm by Tissue culture plate method. Detection of icaAB, atlE genes which code for polysaccharide intracellular adhesin (PIA) that is fundamental for biofilm formation and mecA gene which encodes an altered Penicillin binding protein (PBP2a) mediating oxacillin resistance was done by multiplex PCR. Results: Out of the 50 cases from clinical samples, 21/50 (42%) of the isolates were from blood, 18/50 (36%) from pus, 9/50 (18%) from urine and 2/50 (4%) were from CSF. S. epidermidis was the most common species isolated among clinical and commensal isolates. Biofilm was detected in 16/50 (32%) clinical isolates and 05/50 (10%) of controls. 37/50 (74%) cases and 27/50 (54%) controls were tested positive for mecA gene. 17/50 (34%) cases and 15/50 (30%) controls were found to express atlE gene. 20/50 (40%) cases and 12/50 (24%) controls possessed icaAB gene. Conclusion: Species identification, antimicrobial susceptibility testing and understanding the genetic components involved in pathogenesis are important aids in the diagnosis and management of serious CoNS infections.
Authors and Affiliations
Faiza Samreen, Anjana Gopi, Prachee Bele Kenge, Swati Jain
Keywords
Related Articles
- EP ID EP294928
- DOI 10.18231/2394-5478.2017.0041
- Views 108
- Downloads 0
Microbiological profile and antibiotic sensitivity pattern in sputum cultures-A retrospective study
Introduction Respiratory tract infections RTI are one of the most common cause of infections and are responsible for loss of working hours in affected individuals Many newer antibiotics have been introduced to treat thes...
Control of Pseudomonas aeruginosa blood stream infection outbreak in neonatal intensive care unit by quantitative antibiogram
Introduction: Neonates are very susceptible to Pseudomonas aeruginosa. Single case of Pseudomonas aeruginosa in preterm neonates is a sign for immediate action. Aim: To describe control of an outbreak of Pseudomonas aeru...
Seroprevalence of Hepatitis- A virus in North Karnataka and its changes over a 5 year period
Introduction Worldwide the most common type of acute viral hepatitis is hepatitis A transmitted via the fecaloral route occasionally leading to death due to acute fulminant hepatitis The present study was undertaken to s...
Bacterial profile and antibiotic susceptibility in chronic obstructive pulmonary disease patients with acute exacerbation: A cross sectional study in a tertiary care hospital
Background: The course of Chronic Obstructive Pulmonary Disease (COPD) is characterized by intermittent exacerbations of the disease. The bacterial pathogens contribute a lot in mucus secretions and the acute exacerbatio...
Characterization and antimicrobial susceptibility pattern of non-fermenting gram negative bacilli from various clinical samples in a tertiary care hospital
Background: Non fermenting Gram Negative Bacilli (NFGNB) once considered as contaminants have now emerged as a major cause of life threatening nosocomial infections and as multidrug resistant pathogens. Aim: To isolate a...