COMPARISON OF PROPAGATION METHOD IN IN VITRO AND IN VIVO CONDITION OF Lonicera caerulea L.
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2017, Vol 42, Issue 334
Abstract
The aim of the study was to determine the effectiveness of rooting of blue honeysuckle of ‘Wojtek’ cultivar in vitro. Moreover, suitability of different soil substrate for rooting the lignified herbaceous cuttings in vivo was evaluated. The process of disinfection was the decisive factor in the process of preparing the explants for the propagation. Among the disinfection solutions used for sterilization of ‘Wojtek’ microshoots, the best results were obtained after application of 10% NaOCl solution. Initiation and propagation of blue honeysuckle proceed with the highest efficiency in Murashige and Skoog (MS) medium, and no positive effect of BAP cytokine on this process was reported. Proper selection of composition of the medium guaranteed high performance of rooting of explants in vitro – lack of plant growth regulators (PGRs) in MS medium led to a decrease in the percentage of rooted plants. The addition of auxins to MS medium had a stimulating effect on plant height, length, and number of roots. In the greenhouse, four-node lignified cuttings in the medium prepared with perlite and peat were rooted with the highest performance. Rooting of lignified herbaceous cuttings in the substrate prepared with peat and perlite stimulated the development of the root system as well. The worst influence for rooted cuttings was observed in sand.
Authors and Affiliations
Marcelina KRUPA-MAŁKIEWICZ, Ireneusz OCHMIAN, Miłosz SMOLIK, Krystyna Maria OSTROWSKA
Keywords
Related Articles
- EP ID EP411432
- DOI -
- Views 97
- Downloads 0
OXYGRAPHIC EVALUATION OF ACTIVITY OF RESPIRATORY ENZYMES IN BOAR'S SEMEN
In the article advantages of the use of oxygraphic method for metabolic examination of mitochondrial respiratory enzymes in boar spermatozoa were described. Investigations were performed on semen samples taken from 36 bo...
ANALYSIS OF THE EFFECT OF THE ORDER OF CALVING, CALF SEX AND MILK YIELD OF COWS ON GROWTH PARAMETERS OF LIMOUSIN CALVES
The study was taken in the farm located in West Pomerania province where Limousine cattle in purity of breed were kept. The study included a total of 226 Limousine calves in purity of breed, which were born between 2011...
THE USE OF FLUORESCENCE SPECTROSCOPY TO ASSESS THE DEGREE OF OVERHEATING OF HONEY
The honey heating process used for its recrystallization (especially in a high temperatures and for a very long time) can result a chemical changes. The aim of this study was to use fluorescence spectroscopy to assess th...
DEPENDENCIES BETWEEN PIT-1 GENE POLYMORPHISM AND PRODUCTION PROGRESS IN POLISH HOLSTEIN-FRIESIAN BLACK-AND-WHITE COWS
The aim of this study was to analyse the dependence between PIT-1 gene polymorphism in exon 6 of bovine chromosome 1 (c.1178G>A) and the level of production progress in Polish Holstein-Friesian Black-and-White cows. The...
ANALYSIS OF FLUORIDE, DRY MATTER CONTENTS AND pH IN SEDIMENTS AND SURFACE WATER OF NOWOGARDZKIE LAKE
The aim of study was to determine the impact of adjacent terrain usage on the fluoride content in sediments and others important ecological indicators. All of results applies to bottom sediments and surface water from No...