Comparison of Semi-Quantitative RPR Test and TPHA for Serodiagnosis of Syphilis
Journal Title: National Journal of Community Medicine - Year 2017, Vol 8, Issue 9
Abstract
Introduction: A reactive RPR should always be confirmed with trepo-nemal test like TPHA in suspected syphilis patients to rule out biologi-cal false positive cases. It is im-portant to find an alternative to TPHA as it is frequently not availa-ble in resource limited health care facilities of developing nations like India. Aim: Aim of our study is to evaluate semi-quantitative RPR test and TPHA in serological diagnosis of syphilis in resource limited health care facilities. Methodology: A retrospective cross-sectional study from July 2012 to July 2015 was conducted on 216 suspected cases of syphilis. All cas-es were tested for qualitative RPR test, semi-quantitative RPR test ant TPHA test. Serum samples that are positive in qualitative RPR test but negative in TPHA were referred to as biologic false-positive (BFP) reac-tions. Statistical analysis was done by using Fischer’s exact test. Results: We found 30(13.88%) bio-logical false positive (BFP) cases in dilutions 1:8 or below on semi quantitative RPR test (P=0.0039). No BFP case was found in dilutions 1:16 or more. (P=0.0177).BFP can occur in any age group. We have noted that female 20(66.66%) were showing more BFP cases in our study (P=0.0009). Conclusions: No biological false positive reaction has been found in above 1:8 dilution of RPR test. Semi-quantitative RPR test results in 1:16 or more dilution is equivalent to TPHA results for diagnosis of syph-ilis.
Authors and Affiliations
Nasiruddin M Shaikh, Tanmay K Mehta, Parul D Shah
Keywords
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