Comparison of the efficacy of calcium hydroxide in association with different proton pump inhibitors against Enterococcus faecalis- An in vitro study.
Journal Title: International Journal of Medical Science and Innovative Research (IJMSIR) - Year 2018, Vol 3, Issue 9
Abstract
Background & Aims: The viability of E. faecalis in infected root dentine has shown to be not completely affected by Calcium hydroxide. The aim of this study was to verify whether the association of proton pump inhibitors- omeprazole, lansoprazole and rabeprazole could increase the antimicrobial efficacy of calcium hydroxide against E. faecalis. Methods: The Zone of Inhibition of Calcium hydroxide along with the proton pump inhibitors was measured against E. Faecalis (ATCC 29212) by well diffusion method. E. faecalis (ATCC 29212) was maintained on BHI broth and cultures of E. faecalis were grown overnight at 37°C in BHI broth for 24 h and inoculated on agar plate. The suspension of calcium hydroxide with water (for control) or with different concentrations of PPI’s (25,20,15,10,5 ug/ml) was placed into the wells made in the agar plates. The plates were allowed to stand for 30 min and then incubated at 37 ± 1°C for 48 h. After 48 h the results were expressed as diameter of the clearing zone around the well measured in millimeters. Results: At 15,20 and 25ug/ml concentration, there was significant difference between the effectiveness of calcium hydroxide with proton pump inhibitor and calcium hydroxide alone. Lansoprazole has shown significantly better results as compared to Omeprazole and Rabeprazole at 25,20,15 and 10ug/ml. Conclusions: Under the conditions of this study, calcium hydroxide in association with Omeprazole, Lansoprazole and Rabeprazole have shown effective results in comparison with calcium hydroxide alone, and show a promising future in the elimination of Enterococcus Faecalis.
Authors and Affiliations
Dr Kavita Dube
Keywords
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