Comparison of the efficiency of the DNA extraction methods of some selected plant species
Journal Title: Scholars Academic Journal of Biosciences - Year 2015, Vol 3, Issue 7
Abstract
Abstract: DNA extraction is an important aspect of plant molecular biology and the current studies require large amount of purified high molecular weight DNA with high quality. The choice of DNA extraction methods explained by Bousquet et al., Cheng et al., Doyle et al., and Michiels et al. with appropriate quantity and quality of DNA extracted from different plant species (Oryza sativa, Nicotiana tabacum, Hibiscus esculentus, Populus alba and Pinus sylvestris) grown in northern region of Sri Lanka were practiced. Based on the quantity and quality of the extracted DNA tested by measuring the absorbance of DNA at 260 nm using Nanodrop® ND-1000 spectrophotometer and measuring the ratio of A260 / A280, the efficiency of the extraction method chosen varies among plant species. Among the methods used, the method introduced by Cheng et al, yielded good and amplifiable quality DNA with satisfactory concentration for all the plant species tested. Keywords: DNA Extraction, Nanodrop, PCR amplification, quality and quantity.
Authors and Affiliations
Ranganathan Kapilan
Keywords
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