Comparison of Two Analytical Methods used for the Measurement of Total Antioxidant Status
Journal Title: Journal of Antioxidant Activity - Year 2014, Vol 1, Issue 1
Abstract
Background: Antioxidants play an important role in maintenance of human health and prevention of disease. Effective supplementation of antioxidants requires laboratory monitoring of antioxidant status. An understanding of the methods used to determine the TAS helps in better interpretation of values obtained using a particular method and also to select a suitable method. Material and Methods: Forty subjects including 25 healthy volunteers and 15 patients diagnosed with rheumatoid arthritis were studied. All samples were analysed for TAS using Ferric reducing ability of plasma (FRAP) method and Trolox equivalent antioxidant capacity (TEAC) assay. Results: Mean TAS values obtained by TEAC method were higher than those obtained by FRAP method (p<0.0001); no difference was observed when TEAC values were corrected for proteins and FRAP values were corrected for uric acid (p=0.420). No correlation was found between TEAC and FRAP methods (p=0.102). However, when TEAC was corrected for proteins, positive correlation was observed with FRAP (p=0.044). There was agreement between the two methods when TEAC values were corrected for proteins. Conclusion: Although the reaction conditions differ, similar compounds react in both the assays and thus TEAC and FRAP assays are comparable. However, the two methods differ with respect to –SH groups and uric acid contributions. This contributes to the higher TAS values obtained by TEAC assay. Thus, in conditions with altered protein or uric acid levels, the two methods may not be used interchangeably. The TEAC assay is to be corrected for protein for comparison of reports of the two assays.
Authors and Affiliations
P. V. L. N. Srinivasa Rao, V. S. Kiranmayi, P Swathi, L. Jeyseelan, M. M. Suchitra, Aparna R Bitla
Keywords
Related Articles
- EP ID EP425937
- DOI 10.14302/issn.2471-2140.jaa-14-617
- Views 169
- Downloads 0
The Potentials of Antioxidant Micronutrients in the Management of Metabolic Syndrome
There is increasing evidence of the prevalence manifestations of metabolic syndrome worldwide. Metabolic syndrome is a cluster of abnormalities characterized by hypertension, central obesity, insulin resistance, endothel...
Anti-Oxidant Phytochemicals as Potential Treatments for Age-Related Macular Degeneration
Age-related macular degeneration (AMD) is responsible for a substantial proportion of severe visual impairment and blindness in people over 50 years of age. Current treatments for AMD are not effective in all patients an...
The Antioxidant and Hepatoprotective Activities of Two Tea Polysaccharides
In the present study, we investigated the chemical compositions, in vitro antioxidant and in vivo hepatoprotective activities of two tea polysaccharides (TPS), which were extracted from two different tea cultivars, Yings...
Some Aspects of Regulatory Criteria for Metal-ligand Homeostasis in Epidermal Cells
The work is dedicated to the problem of the norm in the quantitative evaluation of metal content in the epidermal cells (hair) obtained by the method of spectrometry. Authors have analyzed the hair samples for Zn, Cu, an...
Antioxidant Protection of Donor Packed Red Blood Cells using Mexidol
The current research shows the possibility of long-term conservation of the erythrocytes an-tioxidant activity in the process of donor packed red blood cells while introducing mexidol to the hemoconservant glugicir compo...