Comparison of two quantitative methods of microscopic feto-maternal haemorrhage evaluation
Journal Title: Postępy Nauk Medycznych - Year 2012, Vol 25, Issue 7
Abstract
<b>Introduction.</b> Quantification of feto-maternal haemorrhage (FMH) is essential for determination of an accurate dose of anti-RhD Ig in immunoprophylaxis of haemolytic disease of foetus and newborn in RhD negative mothers. The Kleihauer-Betke (K-B) test is one of some methods introduced to our laboratory for FMH investigation. In some countries it has been used routinely for many years, but in Poland only occasionally. K-B test is based on the different properties of haemoglobin F (HbF) in fetal red blood cells (RBCs) and haemoglobin A (HbA) in maternal RBCs. HbF is more resistant than HbA to both alkali denaturation and acid elution.<br><b>Material and methods.</b> Dried and stained blood films were examined using two quantitative methods: in the 1<sup>st</sup> of them we counted twice (2 slides) foetal RBCs between 1000 maternal “ghost” cells, in the 2<sup>nd</sup> we evaluated at least 10 000 cells, using a Miller square. These methods were performed 10 times for each prepared mixtures with 0.1%, 0.25%, 0.5%, 1% and 2% of foetal RBCs in adult blood, imitating FMH.<br><b>Results.</b> Coefficients of variation (CV) were appropriate in the 2<sup>nd </sup>method (< 20%) and estimated volumes of FMH were accurate. These values were not proper using the 1<sup>st</sup> method (CV about 30% to 64%).<br><b>Conclusions. </b>K-B test can be the sensitive method for quantification of FMH if we assess at least 10 000 RBCs.
Authors and Affiliations
Agata Gieleżyńska, Jadwiga Fabijańska-Mitek
Keywords
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