Comparison of Two Spectrophotometric Methods for Quantifying Total Hydroxycinnamic Acids in Coneflower (Echinacea purpurea) Preparations
Journal Title: Journal of Herbal Drugs - Year 2018, Vol 8, Issue 4
Abstract
Background & Aim: Hydroxycinnamic acids are one of the most important bioactive substances of Echinacea drugs. These compounds possess immuno-enhancing activity and thus, total hydroxycinnamic acids are mostly used as the main criterion for quality control of Echinacea purpurea and its drugs. Hence, the quality control of Echinacea requires to developing the reliable methods which are simple and also have a suitable accuracy in measuring total hydroxycinnamic acids. The current study was performed in order to sets a simple method for quantifying total hydroxycinnamic acids of Echinacea and then, comparison of this method which named as the AlCl3 method with the method of European pharmacopoeia (EP). Experimental: Total hydroxycinnamic acids of 10 coneflower samples were determined using AlCl3 and EP methods and the amounts obtained by these two methods, were compared using statistical criterion. Results: Accuracy of AlCl3 method in measuring total hydroxycinnamic acids of Echinacea was not suitable, as the relative difference between amounts measured by this method and those measured by EP method was 50-130%. Comparison of this method with EP method indicated that performance of AlCl3 method for quantifying total hydroxycinnamic acids of Echinacea drugs was very poor and low value of willmott index of agreement (d= 0.53) and high value of relative error (RE= 0.83) were obtained. Recommended applications/industries: Due to non-selective reaction of Al(III) with hydroxycinnamic acids, the AlCl3 method is not suitable for measuring the total hydroxycinnamic acids of Echinacea and its preparations.
Authors and Affiliations
Hossein Mahdavi; Mohammad Reza Naderi; Mohammad Hossein Esmaeili
Keywords
Related Articles
- EP ID EP23561
- DOI http://dx.doi.org/10.14196/JHD.%202018.%20235
- Views 431
- Downloads 20
Effect of different growth regulators and wound treatment in increasing rooting of Myrtus Communis cuttings
مقدمه و هدف: استفاده از قلمه، یکی از مهمترین شیوههای افزایش درختان و درختچهها میباشد. گیاه مورد ((Myrtus communis L. یکی از گیاهان دارویی و بومی فلور ایران و از گیاهان ویژه منطقه مدیترانه است که در مناطق استپی با شرا...
Genetic improvement of essence percent and dry flower yield using indirect selection in German chamomile (Matricaria chamomilla L.)
Background & Aim: German chamomile is one of the most important medicinal plants. So, assessment of genetic diversity and relationship among the traits are very essential in chamomile breeding programs. Determination of...
Interactive effects of salicylic acid and cold stress on activities of antioxidant enzymes in Glycyrrhiza glabra L.
Background & Aim: Glycyrrhiza L. (Fabaceae) consists of perennial herbs grow in sandy soils with hard lightness. Three species of the genus grow in Iran, among them G. glabra L. has the widest distribution. It is well kn...
The phytochemical response of Melissa officinalis L to foliar application of hydro-alcoholic solutions (methanol and ethanol)
Introduction & Aim: The aim of this study was evaluating effect of methanol and ethanol foliar application as a new and safe technology in plant production on lemon balm’s (Melissa officinalis L.) volatile oil components...
Antidiabetic and hypolipidaemic activities of Solenostemon monostachyus
Background & Aim: Solenostemon monostachyus has remain invincible in Ibibio ethnomedicine for the management of numerous ailment including diabetes mellitus. Many of these ethnomedicinal claims are yet to be pharmacol...