Complex selection in vitro of sugar beet (Beta vulgaris L.) for droughtproof and solismity
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2018, Vol 10, Issue 1
Abstract
In recent years, the development of crop production is aimed at creating, capable of responding quickly to stressful effects and subsequent self-regulation of agro-ecosystems. One of the promising directions that make it possible to create the source material resistant to extreme conditions and increase the efficiency of the formation of new forms of plants is the use of in vitro cell selection methods. The purpose of the work is to receive cell lines and sugar beet regenerators, drought-resistant and salinized. The research used a culture of isolated cells and tissues and cellular selection. The subletal concentrations of the selective agents for drought tolerance, which represented 20% PEG 6000 and sulfate salinity (2.5%), were established. The complex sublethal concentration was found to be 20% PEG 6000 and 2% Na2SO4, which was used to isolate the cell lines. As a result of the conducted studies, 4% of resistant clones were isolated, which stably maintained a sign of complex resistance. The stability of the signs of stability obtained from the droughtproof lines to the complex of stress factors was determined in the order of using the system of repacking on nutrient media with stress factors and without adding them. The resulting galley lines with complex resistance were characterized by a dense, globular structure with yellowish coloration and marked by very slow growth. The frequency of morphogenic callus formation was 51-56%. The highest ability to regenerate was characterized by the jaw lines of the YalO64 (77%), the triploid hybrid Alexandria (60%) and the diploid UkrMS 70 (50%). Ranking of a collection of sugar beet lines with complex resistance to drought and salinity, according to which the genotypes studied, were grouped into three groups: high, medium and weakly stable. The best results for the investigated varieties and sugar beet hybrids, regardless of genotype, were observed on MC9 medium supplemented with 0.5 mg / L NSC and 0.1 mg / L GC3 for the duration of the rooting process for 11-15 days. The subunit of sugar beet sugar beet regenerants in the substrate, depending on the genotype, ranged from 68 to 80%. Conclusions: The scheme of cell selection in vitro on selective media has been developed and proposed for obtaining stable lines of sugar beet to two stress factors. Cell lines and plant regenerants with complex drought tolerance and sulfate salinity were obtained.
Authors and Affiliations
O. L. Klyachenko
Keywords
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- EP ID EP498717
- DOI 10.31861/biosystems2018.01.014
- Views 94
- Downloads 0
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