INFLUENCE OF CINNOMICAND OXYBENZOIC ACIDS ON METABOLISM AND REGENERATION PROCESSES IN EXPLANTS OF ROSA ESSENTIAL OIL UNDER IN VITRO CONDITIONS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2017, Vol 9, Issue 1
Abstract
The paper highlights the effects of oxybenzoic and cinnomic acids on the regeneration processes in tissues and organs of Rosa essential oil. The research was carried out on plants-regenerates of Lan’ variety of Rosa essential oil. Plant explants were cultured on Quoirin and Lepoivre nutrient media with the addition of phenolic acids at a concentration of 1 mM / l. On the 25th day of cultivation in the leaves of plants-regenerants, the content of phenols, flavonoids, pigments and catechins and antioxidant activity in methanol extract of leaves were determined. It has been shown that vanillic, syringic, ferulic and gallic acids actively influenced the histogenesis and organogenesis in plantsregenerants in millimolar concentrations. All investigated phenolic acids induced callus formation at the basal part of shoots in the areas of direct contact with NM. The greatest stimulation of growth of the shoots was shown by methylated acids. Cinnomic acids acted as inductors of callusogenesis in shoots of Rosa essential oil. In the presence of 4-hydroxy and 3-methoxy groups, phenolic acids stimulated the growth of stems. With an increase in the number of oxygroups (caffeic and gallic acids) of oxybenzoic and cinnomic acids affected the decrease in the amount of chlorophylls in the leaves. Since a significant portion of phenolic compounds is synthesized in chloroplasts and is indirectly associated with photosynthesis processes, it was quite obvious that the least amount of phenols, in particular catechins and flavonoids, were found in the leaves of ROSA that were under influence of caffeic and gallic acids. The most stimulating effect on the phenylpropanoid synthesis had vanillin acid. It has been established that the increase in the number of hydroxyl groups in the molecular structure of oxybenzoic acids contributes to the decrease in the synthesis of common phenols and catechins (gallic acid). The presence of one or two methoxy groups in the structure of benzene ring gives phenolic acids properties to increase the activity of phenylpropanoid synthesis (vanillic, syringic). The presence of substitutes of the first kind in the structure of cinnomic acids weakens their potential impact on the synthesis of phenolic compounds in leaves of Rosa essential oil. It has been proved that oxybenzoic (vanilli, syringic, gallic) and cinnomic (pcoumaric, caffeic, and ferulic) acids in the nutrient medium (conc. of 1 mM / l) in the area of contact with living tissues initiate the callusogenesis. It has been shown that the use of gallic acid in nutrient media in millimolar concentrations may be appropriate at the first stages of introducing the Rosa essential oil plants into in vitro culture to reduce the risk of auto-toxication of tissues by the oxidation of phenols.
Authors and Affiliations
О. О. ОЛІЙНИК, А. Ф. ЛІХАНОВ, М. Д. МЕЛЬНИЧУК
Keywords
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