Construction of cDNA libraries from leaves of Calcutta 4 and Niyarma Yik banana cultivars inoculated with Pseudocercospora fijiensis Morelet
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 1
Abstract
Molecular studies of plant-pathogen interaction are very important for the identification of gene (s) related with the pathogenic process, as well as with the plant resistance. These gene (s) could be use for the genetic improvement programs in order to obtain resistant cultivars. The aim of this work was to construct complementary DNA (cDNA) libraries from infected leaves with Pseudocercospora fijiensis CCIBP-Pf1 isolated of two banana cultivars (a resistant one Calcutta4 and another one susceptible Niyarma Yik). First-strand cDNA synthesis, was made beginning with one microgram of total RNA by using oligo dT primer and cDNA quality was checked by Polimerase chain reaction (PCR) with cytochrome b specific primers. Second-strand cDNA synthesis was performed by using the homopolymeric tailing with dC-BamH I + dT-Not I primer combination. Four cDNA libraries of infected plants at different times of infection with the pathogen were obtained. Forty one clones of one of the libraries of Niyarma Yik were sequenced and the obtained sequences correspond with genes related to fungi.
Authors and Affiliations
Milady F. Mendoza Rodríguez , Elio Jiménez, Frank Maier , Whilhelm Schäfer, Idalmis Bermúdez, Yennys Padrón, Michel Leyva, Yelenys Alvarado, Mayra Acosta, Orelvis Portal
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