Culture medium and growth regulators on in vitro multiplication of Psidium guajava L.
Journal Title: Biotecnología Vegetal - Year 2014, Vol 14, Issue 1
Abstract
Guava (Psidium guajava L.) cultivar ‘Dwarf Cuban Red 18-40 EEA’ has high yields. For large-scale propagation, micropropagation is a possible solution. The aim of this study was to determine the effect of two culture media, two cytokinins and an analog brasinoesteoides (DI-31) in the in vitro multiplication of this cultivar. Two culture media (MS and WPM), three concentrations of benzylaminopurine (BAP) (0.5, 1.0, 1.5 mg l -1 ), three of kinetin (0.5, 1.0, 1.5 mg l -1 ) and two DI-31 (0.01 and 0.02 mg l -1 ) were evaluated. The variables evaluated were: number of shoots, number of leaves, shoot length and multiplication coefficient. It was found that the type of culture medium influenced the shoot multiplication of guava. The number of shoots, shoot length and multiplication coefficient were determined by the type and concentration of cytokinin added to the culture medium. With the use of WPM culture medium with 1 mg l -1 BAP It was obtained the highest values of the variables evaluated. The use of DI-31 promoted the shoot growth without affecting the multiplication coefficient.
Authors and Affiliations
Jorge Vilchez , Leonardo Martínez , Carlos Alvarez , Angel Albornoz , Nilca Albany , Miguel Molina , Leyanis García-Águila
Keywords
Related Articles
- EP ID EP683552
- DOI -
- Views 138
- Downloads 0
An alternative to estimate the retention of atmospheric carbon by Bambusa vulgaris var. Schrad vulgaris. ex J.C. Wendl.
The estimation of atmospheric carbon sequestration by a woody plant has two alternatives for implementation: by identifying the average annual increase in volume or by determining the appropriate method for calculating...
Effect of two plant growth regulators and illumination conditions in the germination of conserved seeds of Clitoria ternatea
The seeds viability lost in the seed legume bank of Research Institute of Pastures and Forages (IIPF) led to the aim of the work it was to determine the effect of two plant growth regulators (gibberellic acid; GA 3 and n...
Agrobacterium tumefaciens-mediated genetic transformation of embryogenic cell suspensions of the banana hybrid cultivar FHIA 21 (AAAB)
The genetic improvement of plantain and banana is of great importance for its level of consumption on a world-wide scale. Genetic transformation constitutes one alternative for genetic improvement and has complemented tr...
In vitro multiplication of Paeonias sp. variety ‘SeSu’ in Temporary Immersion Systems
Paeonia are considered among the most important perennial ornamentals. The influence of three culture methods (semi-solid culture medium, static liquid culture medium and liquid culture medium by temporary immersion) a...
Extraction of anthocyanins from black corn cultivated in Tunshi- Chimborazo subjected to different heat treatments for scalding
In order to avoid the loss of anthocyanins during the preparation of black corn (Zea mays L.) as food, and for the extraction of these metabolites for industrial purposes, it is necessary to determine the effect of seve...