Conventional and rapid methods for identification of Staphylococcus aureus from clinical specimens

Journal Title: Scientific Journal of Microbiology - Year 2013, Vol 2, Issue 9

Abstract

Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium whose incidence ranges from skin, soft tissue, respiratory, bone, joint, endovascular to wound infections. The purpose of this study was to identify Staphylococcus aureus from clinical specimens using routine conventional and rapid tests. Gram staining, catalase test, coagulase test, DNase test, haemolysis on blood agar and Microgen™ STAPH-ID kit tests were carried out. A total of 125 Gram positive cocci were tested. The Gram staining technique yielded 100 (80.00%) Staphylococcus spp (Gram positive cocci in clusters). 89(71.20%) isolates were positive to haemolysis on blood agar. Mannitol Salt Agar, DNase agar and Catalase test correctly identified 69 (55.2%) of the Gram positive cocci to be S. aureus as was confirmed by the Microgen™ STAPH-ID kit test. Coagulase test yielded 66 (52.8%) positive results. The Microgen™ STAPH-ID kit test identified three non-coagulase Staphylococcus aureus isolates. The Microgen™ STAPH-ID kit test was the most reliable of the tests, with accuracy comparable to any other rapid test. However, it is the most expensive of the tests. This study established that conventional tests can be used for direct identification of S. aureus to species level if the battery of tests is increased.

Authors and Affiliations

N. C. J. Anyanwu*| Department of Microbiology, Faculty of Sciences, Ahmadu Bello University, Zaria, Nigeria., W. C. John| Federal College of Forestry, Jos, Plateau State, Nigeria.

Keywords

Related Articles

Uropathogens from diabetic patients with asymptomatic bacteriuria and Urinary tract infections

Urinary tract infections occur more frequently in diabetic than in non-diabetic patients and have been proven to be the primary cause of renal failure in diabetics. This study was to investigate the prevalence of asympto...

Isolation, identification and antibiogram profiles of Staphylococcus aureus from commercial broiler flocks in Dinajpur District of Bangladesh with special focus on the determination of lethal effect of extracted toxin

The present study was designed with a view to isolate, identifies and characterizes Staphylococcus aureus from commercial broiler flocks having typical symptoms in Dinajpur District of Bangladesh with special focus on th...

Bio-efficacy of different antibacterial antibiotic, plant extracts and bioagents against bacterial blight of soybean caused by Pseudomonas syringae pv. glycinea

An experiment was carried out to study efficacy of different antibacterial antibiotic, plant extracts and bioagents against bacterial blight of soybean caused by Pseudomonas syringae pv. glycinea. The results revealed th...

Management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum

An experiment was carried out to see the efficacy of different chemicals and bioagents against bacterial blight disease severity (PDI) and disease incidence (PI). Significantly low disease severity and low disease in...

Isolation of microorganisms and screening of heavy metals from Municipal Council’s treated sewage from Nakuru sewage treatment plant in Nakuru County, Kenya

Diarrhoeal cases due to taking of contaminated water are on the increase Nakuru County. They are a leading cause of human mortality and morbidity. The current study was aimed at isolating microorganisms from Municipal’s...

Download PDF file
  • EP ID EP1172
  • DOI 10.14196/sjm.v2i9.787
  • Views 541
  • Downloads 25

How To Cite

N. C. J. Anyanwu*, W. C. John (2013). Conventional and rapid methods for identification of Staphylococcus aureus from clinical specimens. Scientific Journal of Microbiology, 2(9), 174-178. https://europub.co.uk/articles/-A-1172